肝炎症和肝纤维化：治疗棘球蚴病相关肝损伤的潜在靶点。
Hepatic inflammation and liver fibrogenesis: A potential target for the treatment of cystic echinococcosis-associated hepatic injury.
机构信息
Laboratory of Cellular and Molecular Biology, department of biology, University of Sciences and Technology Houari Boumediene, Algiers Algeria.
Department of Anatomy and Pathological Cytology, University Hospital Center Mustapha Pacha, Algiers Algeria.
出版信息
Acta Trop. 2022 Feb;226:106265. doi: 10.1016/j.actatropica.2021.106265. Epub 2021 Dec 9.
To investigate the effect of cystic echinococcosis (CE) on liver damage, we developed a secondary experimental echinococcosis in Swiss mice by intraperitoneal inoculation of viable protoscoleces. Mice were randomly allocated into three groups: Ctrl group, PBS group, and CE group. Mice were euthanized and associated indications were investigated to evaluate inflammatory and fibrotic responses in liver. Hepatic damage and fibrotic reaction were histologically analyzed. The hepatic expression of iNOS, TNF-α, NF-κβ, vimentin, Bcl-2 and CD68 was evaluated by Immunohistochemical examinations. Interestingly, a significant iNOS, TNF-α, NF-κβ, vimentin, Bcl-2 and CD68 increase levels was observed in liver tissue and pericystic layer of hepatic hydatid cyst and correlate with the abundance of collagen and reticulin fibers. These observations could promote a potential target for the treatment of CE-associated hepatic injury.
为了研究肝包虫病（CE）对肝脏损伤的影响，我们通过腹腔接种活原头蚴在瑞士小鼠中建立了继发性实验性包虫病。将小鼠随机分为三组：Ctrl 组、PBS 组和 CE 组。处死小鼠并检测相关指标，以评估肝脏的炎症和纤维化反应。对肝损伤和纤维化反应进行组织学分析。通过免疫组织化学检查评估肝脏中 iNOS、TNF-α、NF-κβ、波形蛋白、Bcl-2 和 CD68 的表达。有趣的是，在肝包虫囊肿的肝组织和囊壁层中观察到 iNOS、TNF-α、NF-κβ、波形蛋白、Bcl-2 和 CD68 水平显著增加，并与胶原和网状纤维的丰度相关。这些观察结果可能为治疗 CE 相关肝损伤提供了一个潜在的治疗靶点。
Zotero 插件