Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Acta Trop. 2020 Mar;203:105306. doi: 10.1016/j.actatropica.2019.105306. Epub 2019 Dec 28.
Cystic echinococcosis (CE) induces in the human host innate and adaptive immune response that plays an important role in controlling the immunopathogenesis. Due to the crucial role of nuclear factor kappa B (NF-κB) in regulating immuno-inflammatory processes, we investigated its potential contribution in systemic and local immuno-inflammatory responses in primary CE patients and relapsed patients. The expression of NF-κB and inducible nitric oxide synthase (iNOS) was analyzed in peripheral blood mononuclear cells (PBMC) as well as in pericystic layer of pulmonary hydatid cysts from Algerian primary CE patients and relapsed patients. Tumor necrosis factor alpha (TNF-α) and nitric oxide (NO) production was evaluated in plasma samples. Our results showed high iNOS and NF-κB expression in both PBMCs and pericystic histiocytes from primary CE patients. In addition, substantial amounts of systemic NO and TNF-α were detected in the same patients. Remarkably, relapsed patients exhibited a low NF-κB and iNOS expression associated with low amounts of plasmatic TNF-α and NO. Collectively, NF-κB/iNOS pathway is involved in the host defense mechanisms at the systemic and local level during primary CE. Our results indicate that the inhibition of this pathway in relapsed patients will attenuate protective immunity and promote parasite escape. This study allowed to identify a novel predictive biomarkers of hydatidosis.
包虫病（CE）诱导人类宿主产生先天和适应性免疫反应，在控制免疫发病机制方面发挥着重要作用。由于核因子 kappa B（NF-κB）在调节免疫炎症过程中起着至关重要的作用，我们研究了它在原发性 CE 患者和复发性患者的全身和局部免疫炎症反应中的潜在作用。分析了阿尔及利亚原发性 CE 患者和复发性患者外周血单个核细胞（PBMC）以及肺包虫囊肿囊壁组织中 NF-κB 和诱导型一氧化氮合酶（iNOS）的表达。评估了血浆样本中肿瘤坏死因子-α（TNF-α）和一氧化氮（NO）的产生。我们的结果表明，原发性 CE 患者的 PBMC 和囊壁组织中的 iNOS 和 NF-κB 表达均较高。此外，在同一患者中还检测到大量的全身性 NO 和 TNF-α。值得注意的是，复发性患者的 NF-κB 和 iNOS 表达较低，与血浆 TNF-α和 NO 的含量较低有关。综上所述，NF-κB/iNOS 通路参与了原发性 CE 期间全身和局部水平的宿主防御机制。我们的研究结果表明，抑制复发性患者的这条通路会减弱保护性免疫并促进寄生虫逃逸。本研究鉴定出了包虫病的一种新的预测性生物标志物。
