功能性促甲状腺激素受体在小胶质细胞中的表达。
Expression of functional thyroid-stimulating hormone receptor in microglia.
机构信息
Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, PR China.
Department of Blood transfusion, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, PR China.
出版信息
Ann Endocrinol (Paris). 2022 Feb;83(1):40-45. doi: 10.1016/j.ando.2021.11.009. Epub 2021 Dec 9.
OBJECTIVE
The purpose of the present study was to clarify the expression of thyroid-stimulating hormone receptor (TSHR) in microglial cells, and to explore its function.
MATERIALS AND METHODS
Expression of TSHR in microglia was determined by Western blot, immunocytofluorescence and double immunohistofluorescence. Cyclic adenosine 3',5'-monophosphate (cAMP) production was measured after thyrotropin receptor stimulating antibody (TSAb) treatment.
RESULTS
Results showed that TSHR protein was expressed and mainly located in the mouse microglia membrane. Moreover, TSAb stimulated cAMP production in mouse microglia (P<0.05).
CONCLUSIONS
This study demonstrated the presence of TSHR in microglial cells. Brain TSHR was able to respond specifically to TSAb stimulation, suggesting that TSHR expression is functional. As microglia are innate immune cells that maintain environmental stability in the central nervous system and play a key role in many neuroimmune diseases, expression of functional TSHR in microglia has important pathophysiological implications.
目的
本研究旨在阐明甲状腺刺激激素受体（TSHR）在小胶质细胞中的表达，并探讨其功能。
材料与方法
通过 Western blot、免疫细胞荧光和双重免疫荧光检测小胶质细胞中 TSHR 的表达。用促甲状腺激素受体刺激抗体（TSAb）处理后，测量环磷酸腺苷（cAMP）的产生。
结果
结果表明 TSHR 蛋白表达并主要位于小鼠小胶质细胞膜上。此外，TSAb 刺激小鼠小胶质细胞中 cAMP 的产生（P<0.05）。
结论
本研究证明了 TSHR 存在于小胶质细胞中。脑 TSHR 能够特异性地对 TSAb 刺激作出反应，表明 TSHR 表达具有功能性。由于小胶质细胞是中枢神经系统中维持环境稳定的固有免疫细胞，并且在许多神经免疫疾病中发挥关键作用，因此小胶质细胞中功能性 TSHR 的表达具有重要的病理生理学意义。
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