School of Water Resource and Environment, Research Center of Environmental Science and Engineering, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), 29 Xueyuan Road, Haidian District, Beijing 100083, People's Republic of China.
School of Water Resource and Environment, Research Center of Environmental Science and Engineering, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), 29 Xueyuan Road, Haidian District, Beijing 100083, People's Republic of China.
J Hazard Mater. 2022 Mar 5;425:127945. doi: 10.1016/j.jhazmat.2021.127945. Epub 2021 Dec 2.
Microbes are important component in terrestrial ecosystem, which are believed to play vital roles in biogeochemical cycles of metalloids in mining and smelting surroundings. Many studies on microbial diversity and structures have been investigated around mining and smelting sites, whereas the ecological processes and co-occurrence patterns that influence the biogeographic distributions of microbial communities is yet poorly understood. Herein, microbial biogeography, assembly mechanism and co-occurrence pattern around mining and smelting zone were systematically unraveled using 16S rRNA gene sequencing. The 66 microbial phyla co-occurring across all the samples were dominated by Proteobacteria, Chloroflexi, Acidobacteria and Crenarchaeota. Obvious distance-decay (r = 0.3448, p < 0.001) of microbial community was observed across geographic distances. Differences in microbial communities were driven by the joint impacts of soil factors, spatial and metalloids levels. Dispersal limitation dominated the microbial assemblies in whole, SC and GX sites while homogeneous selection governed that in YN site. The changes in pH and Sb level significantly influenced the deterministic and stochastic processes of microbial communities. Network analysis suggested a typical module distribution, which had apparent ecological links among taxa in modules. This study provides first insight of the mechanism to maintain microbial diversity in metalloids-laden biospheres.
微生物是陆地生态系统的重要组成部分，它们被认为在采矿和冶炼环境中类金属的生物地球化学循环中起着至关重要的作用。围绕着采矿和冶炼场地，已经有许多关于微生物多样性和结构的研究，但影响微生物群落生物地理分布的生态过程和共存模式仍知之甚少。在此，我们使用 16S rRNA 基因测序系统地揭示了采矿和冶炼区周围的微生物生物地理学、组装机制和共存模式。在所有样本中共同存在的 66 个微生物门主要由变形菌门、绿弯菌门、酸杆菌门和泉古菌门组成。跨地理距离的微生物群落明显存在距离衰减（r=0.3448，p<0.001）。土壤因素、空间和类金属水平的共同影响导致了微生物群落的差异。在整个、SC 和 GX 采样点，扩散限制主导了微生物的组装，而在 YN 采样点，均匀选择主导了微生物的组装。pH 值和 Sb 水平的变化显著影响了微生物群落的确定性和随机性过程。网络分析表明存在典型的模块分布，模块中的分类群之间存在明显的生态联系。本研究首次深入了解了维持富含类金属生物圈中微生物多样性的机制。
