Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Nutritional Quality and Safety of Agro Products, Wuhan 430064, Hubei, PR China.
Technology Center of Wuhan Customs District, Wuhan 430036, Hubei, PR China.
Food Chem. 2022 Apr 16;374:131761. doi: 10.1016/j.foodchem.2021.131761. Epub 2021 Dec 2.
A rapid, selective, and sensitive method was developed for the detection of carbendazim and thiabendazole in edible vegetable oil. Two benzimidazole analytes were pre-concentrated by magnetic solid phase extraction (MSPE) using flowerlike Ni-NiO composite as sorbents and followed by LC-MS/MS analysis. The flowerlike Ni-NiO composite sorbent displayed a high affinity towards benzimidazole analytes due to the reversible coordination interaction between the Ni(Ⅱ) ion and the electron-donating imidazole group. In comparison to the previous methods, this procedure is less time-consuming and simpler during sample preparation. The parameters affecting the extraction efficiency were optimized in detail. The method was validated according to SANTE/12682/2019. The limits of detection were in the range of 0.001-0.003 mg•kg. The recoveries ranged from 89.3% to 110.7% with inter-day and inter-day precision less than 10.9%. The results indicate that flowerlike Ni-NiO composite might be a promising alternative for MSPE of benzimidazole compounds in foods.
建立了一种快速、选择性和灵敏的方法，用于检测食用油中的多菌灵和噻菌灵。采用花状 Ni-NiO 复合材料作为吸附剂，通过磁固相萃取（MSPE）对两种苯并咪唑分析物进行预浓缩，然后进行 LC-MS/MS 分析。花状 Ni-NiO 复合材料吸附剂对苯并咪唑分析物具有高亲和力，这是由于 Ni(Ⅱ)离子与供电子的咪唑基团之间的可逆配位相互作用。与以前的方法相比，该方法在样品制备过程中耗时更短、更简单。详细优化了影响萃取效率的参数。该方法按照 SANTE/12682/2019 进行了验证。检测限范围在 0.001-0.003 mg•kg 之间。日内和日间精密度均小于 10.9%，回收率在 89.3%至 110.7%之间。结果表明，花状 Ni-NiO 复合材料可能是食品中苯并咪唑类化合物 MSPE 的一种有前途的替代品。
