Curtin N A, Howarth J V, Rall J A, Wilson M G, Woledge R C
J Muscle Res Cell Motil. 1986 Aug;7(4):327-32. doi: 10.1007/BF01753653.
The heat and force produced in tetanic contraction of single fibres from anterior tibialis muscle of the frog Rana temporaria have been observed at measured temperatures close to 1 and 10 degrees C. Heat was measured using a Hill-Downing type thermopile. In control experiments with a resistor of known heat capacity comparable to a single muscle fibre, it was found that Peltier and Joule heating produced identical thermopile outputs. The Peltier method was used to introduce a known amount of heat into the system in each experiment with a muscle fibre. From the response to this heating the heat capacity of each preparation was obtained and used to calculate the absolute amount of heat production from the thermopile output. The heat produced during tetanic contraction (H) could be described by Aubert's equation [H = Ha (1 - e-t/tau) + hbt]. In some fibres there was no labile heat (Ha), whereas in others it was clearly present. The stable heat rate (hb) was strongly temperature dependent (Q10 = 4.06). At 0 degree C the stable heat rate (normalized by dry weight) in the single fibres was significantly greater than that in whole anterior tibialis muscle.
在接近1摄氏度和10摄氏度的测量温度下，观察了泽蛙胫前肌单纤维强直收缩时产生的热量和力量。热量使用希尔-唐宁型热电堆进行测量。在使用与单根肌纤维热容量相当的已知热容量电阻器的对照实验中，发现珀尔帖热和焦耳热产生相同的热电堆输出。在每次使用肌纤维的实验中，采用珀尔帖方法向系统中引入已知量的热量。根据对这种加热的响应，获得每种制剂的热容量，并用于根据热电堆输出计算产热的绝对量。强直收缩期间产生的热量（H）可用奥伯特方程描述[H = Ha (1 - e-t/tau) + hbt]。在一些纤维中没有不稳定热（Ha），而在另一些纤维中则明显存在。稳定热率（hb）强烈依赖于温度（Q10 = 4.06）。在0摄氏度时，单纤维中的稳定热率（以干重归一化）显著高于整个胫前肌中的稳定热率。
