Department of Cosmetology, Chair of Cosmetology, School of Pharmaceutical Science, Medical University of Silesia, Katowice, Poland.
Department of Nutrigenomics and Bromatology, Chair of Molecular Biology, School of Pharmaceutical Science, Medical University of Silesia, Katowice, Poland.
J Cosmet Dermatol. 2022 Aug;21(8):3561-3566. doi: 10.1111/jocd.14667. Epub 2021 Dec 12.
Keloids arise most often as a result of abnormal wound healing. The lesion characterizes inflammation and fibroproliferation. Fibroblast activation protein alpha (FAP-α) is one of enzymes engaged in keloid formation. It is serine protease that facilitates cells invasion and growth. FAP-α possess also non-enzymatic activity and plays role in activation of cell signaling. The aim of the study was to assess the impact of culture conditions on the proliferation of keloid fibroblasts and the expression of FAP-α. Analysis of utility of 3D models of keloid in in vitro study of pathogenesis of keloids was also made.
NHDF and KEL FIB cells were cultured in vitro in 2D cultures and 3D Matrigel models. The viability of cells was assayed spectrophotometrically with WST-1 test. FAP-α protein amount in cell cultures and 3D models was evaluated with the use of ELISA test.
KEL FIB fibroblasts exhibited higher viability than NHDF fibroblasts in all three models of keloid. The expression of FAP-α is different in normal and keloid fibroblasts. In vitro conditions influence the expression of FAP-α in NHDF cells but not in KEL FIB cells.
This preliminary study has shown that the expression of FAP-α, similarly to other enzymes engaged in keloid formation, is different in keloids in vivo and in in vitro models. FAP-α expression is modulated by in vitro conditions in normal fibroblasts but not in keloid fibroblasts.
瘢痕疙瘩多由异常愈合引起。病变的特征是炎症和纤维增生。成纤维细胞激活蛋白α（FAP-α）是参与瘢痕疙瘩形成的酶之一。它是一种丝氨酸蛋白酶，促进细胞侵袭和生长。FAP-α还具有非酶活性，并在细胞信号转导的激活中发挥作用。本研究旨在评估培养条件对瘢痕疙瘩成纤维细胞增殖和 FAP-α表达的影响。还分析了瘢痕疙瘩 3D 模型在体外研究瘢痕疙瘩发病机制中的应用。
NHDF 和 KEL FIB 细胞在体外 2D 培养和 3D Matrigel 模型中培养。使用 WST-1 试验通过分光光度法测定细胞活力。使用 ELISA 试验评估细胞培养物和 3D 模型中 FAP-α 蛋白的量。
在三种瘢痕疙瘩模型中，KEL FIB 成纤维细胞的活力均高于 NHDF 成纤维细胞。正常和成纤维细胞瘢痕疙瘩中的 FAP-α 表达不同。体外条件会影响 NHDF 细胞中 FAP-α的表达，但不会影响 KEL FIB 细胞。
这项初步研究表明，与参与瘢痕疙瘩形成的其他酶一样，FAP-α 的表达在体内瘢痕疙瘩和体外模型中是不同的。FAP-α的表达在正常成纤维细胞中受体外条件调节，但在瘢痕疙瘩成纤维细胞中不受调节。
