Ying Yong Sheng Tai Xue Bao. 2021 Nov 15;32(11):4129-4138. doi: 10.13287/j.1001-9332.202111.039.
Periphyton is an emerging biological technology for water purification. However, the removal effect of periphyton on antibiotic contaminants in water under different nitrogen and phosphorus levels remains largely unknown. In this study, four nitrogen and phosphorus levels [N-P (mg·L): 2-0.2, 5-0.5, 8-0.8, 11-1.1] were set up to grow periphyton outdoors in plastic crates. The growth, photosynthe-tic activity, species composition, and removal of sulfonamide and enrofloxacin were simulated at a medium scale. The results showed that biomass of the surrounding organisms increased with the increases of culture time. In contrast, the photosynthetic pigment content and photosynthetic activity showed a "single peak" pattern, which first decreased and then increased, indicating that algae in the biofilm would be stressed by antibioics but could quickly adapt and recover vitality. In addition, different nitrogen and phosphorus concentrations resulted in differences in community composition. With the increases of nutrient concentrations, species richness of periphyton algae gradually decreased. The relative abundance of Dictyosphaerium and Chlorella in each treatment was relatively high. Results of 16S rRNA high-throughput sequencing showed that the flora of Rhizobiaceae, Frankiales, and Moraxellaceae was significantly enriched in groups 2-0.2. The relative abundance of Chitinophagaceae in all the four treatments was the highest. The removal rate of sulfonamide in all treatments was higher than 50%, while the removal rate of enrofloxacin in all treatments was more than 90%. The removal rate of sulfonamide in (N-P) 2-0.2 mg·L group (65.8%) was significantly higher than that in other groups, but with no significant difference in the removal rate of enrofloxacin among all treatments. The results showed that periphyton had an excellent ability to remove sulfonamide and enrofloxacin in a wide range of N-P levels. The removal rate of soluble nitrogen in water was not different in each treatment group, and the removal effect of soluble phosphorus was substantial. Our results provided primary data for the ecological removal of sulfonamide and enrofloxacin in water, which gave a new idea for the development of environmental removal technology for new antibiotic pollutants in water.
底栖藻类是一种新兴的水净化生物处理技术。然而，底栖藻类在不同氮磷水平下对水中抗生素污染物的去除效果仍知之甚少。本研究在室外塑料箱中设置了 4 种氮磷水平[N-P（mg·L）：2-0.2、5-0.5、8-0.8、11-1.1]来培养底栖藻类，以中试规模模拟底栖藻类的生长、光合活性、物种组成以及对磺胺类和恩诺沙星的去除效果。结果表明，随着培养时间的延长，周围生物的生物量增加。相反，光合色素含量和光合活性呈现“单峰”模式，先降低后升高，表明生物膜中的藻类会受到抗生素的胁迫，但可以迅速适应并恢复活力。此外，不同的氮磷浓度导致群落组成的差异。随着营养浓度的增加，底栖藻类的物种丰富度逐渐降低。各处理中 Dicyosphaerium 和 Chlorella 的相对丰度相对较高。16S rRNA 高通量测序结果表明，在 N-P 2-0.2 组中 Rhizobiaceae、Frankiales 和 Moraxellaceae 的丰度明显增加。所有处理中 Chitinophagaceae 的相对丰度最高。所有处理中磺胺类的去除率均高于 50%，而所有处理中恩诺沙星的去除率均高于 90%。N-P 2-0.2 mg·L 组（65.8%）磺胺类的去除率明显高于其他组，但所有处理中恩诺沙星的去除率无显著差异。结果表明，底栖藻类在较宽的氮磷水平范围内对磺胺类和恩诺沙星具有良好的去除能力。各组水中可溶性氮的去除率没有差异，而水中可溶性磷的去除效果显著。本研究结果为磺胺类和恩诺沙星在水中的生态去除提供了初步数据，为水中新型抗生素污染物环境去除技术的发展提供了新的思路。
