Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.
Hubei Cancer Clinical Study Center & Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan, China.
Bioengineered. 2021 Dec;12(2):12755-12766. doi: 10.1080/21655979.2021.1997696.
For humans, gastric cancer (GC) is a common malignancy. Multiple circular RNAs (circRNAs) have been confirmed to be important cancer-promoting or tumor-suppressive factors. The present study discusses the roles and mechanisms of circ_0000423 in GC development. In this study, circ_0000423 expression in GC patient tissue samples and cell lines was detected via quantitative real-time polymerase chain reaction. Disheveled-Axin domain containing 1 (DIXDC1) expression in GC cells was examined via Western blot. Besides, cell counting kit-8 was utilized for detecting GC cell viability. GC cell migration and invasion were examined through Transwell assays. Bioinformatics and dual-luciferase reporter gene assays were employed to verify the regulatory relationships between microRNA-582-3p (miR-582-3p) and circ_0000423 or DIXDC1. In the present study, we demonstrated that circ_0000423 was highly expressed in GC. Circ_0000423 knockdown suppressed GC cell viability, migration and invasion. Moreover, miR-582-3p was confirmed as a direct target of circ_0000423, and an upstream regulator of DIXDC1. MiR-582-3p inhibition or DIXDC1 overexpression could reverse the above-mentioned effects of knocking down circ_0000423 on GC cells. In conclusion, circ_0000423 facilitates GC progression by modulating the miR-582-3p/DIXDC1 axis.
对于人类来说，胃癌（GC）是一种常见的恶性肿瘤。已经证实，多个环状 RNA（circRNAs）是重要的促进癌症或肿瘤抑制因子。本研究探讨了 circ_0000423 在 GC 发展中的作用和机制。在本研究中，通过实时定量聚合酶链反应检测 GC 患者组织样本和细胞系中的 circ_0000423 表达。通过 Western blot 检测 GC 细胞中的 Disheveled-Axin 结构域包含 1（DIXDC1）表达。此外，使用细胞计数试剂盒-8 检测 GC 细胞活力。通过 Transwell 测定法检测 GC 细胞迁移和侵袭。通过生物信息学和双荧光素酶报告基因测定法验证 microRNA-582-3p（miR-582-3p）和 circ_0000423 或 DIXDC1 之间的调控关系。在本研究中，我们证明 circ_0000423 在 GC 中高度表达。circ_0000423 敲低抑制 GC 细胞活力、迁移和侵袭。此外，miR-582-3p 被确认为 circ_0000423 的直接靶标，也是 DIXDC1 的上游调节剂。抑制 miR-582-3p 或过表达 DIXDC1 可以逆转敲低 circ_0000423 对 GC 细胞的上述作用。总之，circ_0000423 通过调节 miR-582-3p/DIXDC1 轴促进 GC 进展。
