Experimental Center, Key Laboratory of Traditional Chinese Medicine Classical Theory, Shandong Provincial Key Laboratory of Traditional Chinese Medicine for Basic Research, Shandong University of Traditional Chinese Medicine, Jinan, China.
College of Health Science, Shandong University of Traditional Chinese Medicine, Jinan, China.
Bioengineered. 2022 Jan;13(1):824-833. doi: 10.1080/21655979.2021.2012954.
The aim of the present study was to explore the effects of bone marrow mesenchymal stem cells (BMSCs), combined with polysaccharide (AMP), in an experimental model of ulcerative colitis. BMSCs were first isolated, cultured, and identified by flow cytometry. A rat model of colitis was established by trinitrobenzene sulfonic acid (TNBS) injection. Rats were treated with BMSCs with or without AMP for 1 or 2 weeks. H&E staining was performed to assess the extent of histological injury. IEC-6 and BMSCs were co-cultured and treated with AMP. Cell migration was measured using the Transwell assay, whilst the levels of cytokines in the rat blood samples were detected using ELISA. In addition, cytokine levels in the cell supernatant were measured by microarray. The results showed that BMSCs were successfully isolated. BMSCs treatment could markedly alleviate injury according to histological analysis and regulate inflammatory cytokine production in this rat model of TNBS-induced colitis, where a higher number of BMSCs was found in the intestinal tract, compared to the model. AMP not only potentiated the effects of BMSCs on preventing TNBS-induced colitis but also promoted BMSC homing to the injured tissue and regulated cytokines. Furthermore, BMSCs and AMP promoted the migration of IEC and influenced multiple genes. In conclusion, AMP treatment improved the therapeutic effects of BMSCs on ulcerative colitis, potentially providing a novel clinical treatment strategy for colitis.
本研究旨在探讨骨髓间充质干细胞（BMSCs）与多糖（AMP）联合应用于溃疡性结肠炎实验模型的效果。首先通过流式细胞术分离、培养和鉴定 BMSCs。采用三硝基苯磺酸（TNBS）注射建立大鼠结肠炎模型。用 BMSCs 联合或不联合 AMP 治疗大鼠 1 或 2 周。采用 H&E 染色评估组织学损伤程度。将 IEC-6 和 BMSCs 共培养，并用 AMP 处理。通过 Transwell 测定细胞迁移，采用 ELISA 检测大鼠血样中细胞因子水平。此外，通过微阵列测定细胞上清液中的细胞因子水平。结果表明成功分离了 BMSCs。BMSCs 治疗可明显缓解组织学分析的损伤，并调节这种 TNBS 诱导的结肠炎大鼠模型中炎症细胞因子的产生，与模型组相比，肠道中发现了更多的 BMSCs。AMP 不仅增强了 BMSCs 预防 TNBS 诱导的结肠炎的作用，还促进了 BMSC 向受损组织归巢并调节细胞因子。此外，BMSCs 和 AMP 促进了 IEC 的迁移并影响了多个基因。总之，AMP 治疗改善了 BMSCs 治疗溃疡性结肠炎的疗效，为结肠炎的临床治疗策略提供了新的思路。
