miR-204 通过靶向 FOXK2 调节 LPS 诱导的 A549 细胞损伤。

Mir-204 Regulates LPS-Induced A549 Cell Damage by Targeting FOXK2.

机构信息

Department of Physiology, Changzhi Medical College, Changzhi, Shanxi 046000, China.

Department of Respiratory and Critical Care Medicine, Shanxi Academy of Hospital Sciences, Taiyuan, Shanxi 046000, China.

出版信息

J Healthc Eng. 2021 Nov 30;2021:7404671. doi: 10.1155/2021/7404671. eCollection 2021.

OBJECTIVE

To assess whether miR-204 and HA affect A549 cell injury induced by lipopolysaccharide. . A549 cells were treated with hirsutanol A, and cell damage was induced by LPS followed by analysis of cell proliferation by CCK-8, cell apoptosis by flow cytometry, apoptosis-related protein expression by western blot, downstream target of miR-20 by dual-luciferase reporter gene, and inflammatory factors by ELISA and PCR.

RESULTS

LPS can significantly inhibit the viability of A549 cells, induce cell apoptosis, and promote the release of IL-6, IL-1, and TNF-, while HA pretreatment can target FOXK2 by upregulating miR-204 levels, thereby alleviating apoptosis and promoting cell viability and at the same time inhibiting the release of inflammatory factors by inhibiting the activation of NF-B.

CONCLUSIONS

miR-204 participates in the protection of HA acute lung injury by targeting FOXK2.

目的

评估 miR-204 和透明质酸 (HA) 是否影响脂多糖诱导的 A549 细胞损伤。用胡蔓藤碱 A 处理 A549 细胞，用 LPS 诱导细胞损伤，然后通过 CCK-8 检测细胞增殖，流式细胞术检测细胞凋亡，Western blot 检测凋亡相关蛋白表达，双荧光素酶报告基因检测 miR-204 的下游靶基因，ELISA 和 PCR 检测炎症因子。结果：LPS 可显著抑制 A549 细胞活力，诱导细胞凋亡，并促进 IL-6、IL-1 和 TNF- 的释放，而 HA 预处理可通过上调 miR-204 水平靶向 FOXK2，从而减轻细胞凋亡，促进细胞活力，同时通过抑制 NF-B 的激活抑制炎症因子的释放。结论：miR-204 通过靶向 FOXK2 参与 HA 急性肺损伤的保护。