• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

AP-1将SMAP-1/SMAPs招募至反式高尔基体网络，以促进极化上皮细胞中的分选。

AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia.

作者信息

Wang Shimin, Yao Longfeng, Zhang Wenjuan, Cheng Zihang, Hu Can, Liu Hang, Yan Yanling, Shi Anbing

机构信息

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Cell Architecture Research Institute, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Front Cell Dev Biol. 2021 Nov 25;9:774401. doi: 10.3389/fcell.2021.774401. eCollection 2021.

DOI:10.3389/fcell.2021.774401
PMID:34901019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8655793/
Abstract

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in intestinal epithelia.

摘要

在常规分泌过程中，AP-1与网格蛋白包被组装的协调作用介导了反式高尔基体网络（TGN）上的分泌性分选。在此，我们发现SMAP-1/SMAPs缺陷导致顶端蛋白ERM-1在TGN的基底外侧积累。相反，基底外侧蛋白SLCF-1异常出现在顶端膜上。SMAP-1与TGN上的AP-1共定位。AP-1的完整性是SMAP-1亚细胞定位所必需的。此外，我们发现SMAP-1的缺失减少了细胞质中网格蛋白阳性结构，这表明SMAP-1在TGN上的网格蛋白组装中具有调节作用。功能实验表明，过表达网格蛋白可有效缓解因缺乏SMAP-1导致的胞吐缺陷，证实了SMAP-1在促进TGN上网格蛋白组装中的作用。总之，我们的结果表明，AP-1复合物调节SMAP-1在TGN上的定位，促进网格蛋白组装，以确保肠道上皮细胞中的极化常规分泌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/9d4b30927a01/fcell-09-774401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/81a2e29f3e87/fcell-09-774401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/9d24966f1688/fcell-09-774401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/61f108602254/fcell-09-774401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/916948cfdc1e/fcell-09-774401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/9d4b30927a01/fcell-09-774401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/81a2e29f3e87/fcell-09-774401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/9d24966f1688/fcell-09-774401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/61f108602254/fcell-09-774401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/916948cfdc1e/fcell-09-774401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/8655793/9d4b30927a01/fcell-09-774401-g005.jpg

相似文献

1
AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia.AP-1将SMAP-1/SMAPs招募至反式高尔基体网络，以促进极化上皮细胞中的分选。
Front Cell Dev Biol. 2021 Nov 25;9:774401. doi: 10.3389/fcell.2021.774401. eCollection 2021.
2
The polarized epithelia-specific mu 1B-adaptin complements mu 1A-deficiency in fibroblasts.极化上皮特异性μ1B衔接蛋白可弥补成纤维细胞中μ1A的缺陷。
EMBO Rep. 2002 May;3(5):471-7. doi: 10.1093/embo-reports/kvf092. Epub 2002 Apr 18.
3
SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network.SMAP2是一种新型的ARF GTP酶激活蛋白，它与网格蛋白和网格蛋白组装蛋白相互作用，并在AP-1阳性早期内体/反式高尔基体网络上发挥作用。
Mol Biol Cell. 2006 Jun;17(6):2592-603. doi: 10.1091/mbc.e05-10-0909. Epub 2006 Mar 29.
4
P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes.P4-ATP酶在从反式高尔基体网络和早期内体进行蛋白质转运过程中对AP-1/网格蛋白功能的需求。
Mol Biol Cell. 2008 Aug;19(8):3526-35. doi: 10.1091/mbc.e08-01-0025. Epub 2008 May 28.
5
A mechanism for differential sorting of the planar cell polarity proteins Frizzled6 and Vangl2 at the -Golgi network.在 -Golgi 网络中，Frizzled6 和 Vangl2 平面细胞极性蛋白的差异分拣机制。
J Biol Chem. 2018 Jun 1;293(22):8410-8427. doi: 10.1074/jbc.RA118.001906. Epub 2018 Apr 17.
6
Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes.钠/碘转运体的基底外侧分拣由衔接蛋白 1 网格蛋白衔接复合物介导。
Thyroid. 2022 Oct;32(10):1259-1270. doi: 10.1089/thy.2022.0163. Epub 2022 Aug 16.
7
The clathrin adaptor AP-1A mediates basolateral polarity.网格蛋白衔接蛋白 AP-1A 介导基底外侧极性。
Dev Cell. 2012 Apr 17;22(4):811-23. doi: 10.1016/j.devcel.2012.02.004.
8
Sorting of furin at the trans-Golgi network. Interaction of the cytoplasmic tail sorting signals with AP-1 Golgi-specific assembly proteins.弗林蛋白酶在反式高尔基体网络中的分选。胞质尾部分选信号与AP-1高尔基体特异性组装蛋白的相互作用。
J Biol Chem. 1999 Mar 19;274(12):8199-207. doi: 10.1074/jbc.274.12.8199.
9
Role of adaptor proteins and clathrin in the trafficking of human kidney anion exchanger 1 (kAE1) to the cell surface.衔接蛋白和网格蛋白在人肾阴离子交换蛋白1（kAE1）转运至细胞表面过程中的作用。
Traffic. 2014 Jul;15(7):788-802. doi: 10.1111/tra.12172. Epub 2014 May 5.
10
AP-1 and AP-3 mediate sorting of melanosomal and lysosomal membrane proteins into distinct post-Golgi trafficking pathways.AP-1和AP-3介导黑素体膜蛋白和溶酶体膜蛋白分选进入不同的高尔基体后运输途径。
Traffic. 2008 Jul;9(7):1157-72. doi: 10.1111/j.1600-0854.2008.00745.x. Epub 2008 Apr 9.

引用本文的文献

1
Arfs on the Golgi: four conductors, one orchestra.高尔基体上的Arfs：四位指挥，一个管弦乐队。
Front Mol Biosci. 2025 Jul 31;12:1612531. doi: 10.3389/fmolb.2025.1612531. eCollection 2025.
2
RAB-10 cooperates with EHBP-1 to capture vesicular carriers during post-Golgi exocytic trafficking.RAB-10与EHBP-1协同作用，在高尔基体后胞吐运输过程中捕获囊泡载体。
J Cell Biol. 2025 Apr 7;224(4). doi: 10.1083/jcb.202410003. Epub 2025 Feb 21.

本文引用的文献

1
RTKN-1/Rhotekin shields endosome-associated F-actin from disassembly to ensure endocytic recycling.RTKN-1/Rhotekin 保护内体相关 F-actin 不被解体，以确保内吞体循环。
J Cell Biol. 2021 May 3;220(5). doi: 10.1083/jcb.202007149.
2
Arf1 orchestrates Rab GTPase conversion at the -Golgi network.Arf1在高尔基体网络中协调Rab GTP酶的转换。
Mol Biol Cell. 2021 May 15;32(11):1104-1120. doi: 10.1091/mbc.E20-10-0664. Epub 2021 Mar 31.
3
LET-502/ROCK Regulates Endocytic Recycling by Promoting Activation of RAB-5 in a Distinct Subpopulation of Sorting Endosomes.
LET-502/ROCK 通过促进分选内体中特定亚群的 RAB-5 的激活来调节内吞回收。
Cell Rep. 2020 Sep 22;32(12):108173. doi: 10.1016/j.celrep.2020.108173.
4
Evolving models for assembling and shaping clathrin-coated pits.网格蛋白包被凹陷的组装和塑形的模型不断发展。
J Cell Biol. 2020 Sep 7;219(9). doi: 10.1083/jcb.202005126.
5
Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.网格蛋白重链的可变剪接有助于从有被小窝向有被斑的转变。
J Cell Biol. 2020 Sep 7;219(9). doi: 10.1083/jcb.201912061.
6
An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling.一个 EHBP-1-SID-3-DYN-1 轴在胞吞循环过程中促进膜管状小管的分裂。
PLoS Genet. 2020 May 8;16(5):e1008763. doi: 10.1371/journal.pgen.1008763. eCollection 2020 May.
7
Revealing Functional Crosstalk between Distinct Bioprocesses through Reciprocal Functional Tests of Genetically Interacting Genes.通过遗传相互作用基因的相互功能测试揭示不同生物过程之间的功能串扰。
Cell Rep. 2019 Nov 26;29(9):2646-2658.e5. doi: 10.1016/j.celrep.2019.10.076.
8
Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast.GTPase 激活蛋白的剖析揭示了出芽酵母 COPI 衣被的功能不对称性。
J Cell Sci. 2019 Aug 29;132(16):jcs232124. doi: 10.1242/jcs.232124.
9
Conformational regulation of AP1 and AP2 clathrin adaptor complexes.AP1 和 AP2 网格蛋白衔接蛋白复合物的构象调节。
Traffic. 2019 Oct;20(10):741-751. doi: 10.1111/tra.12677. Epub 2019 Aug 6.
10
Unconventional mechanisms of eukaryotic protein secretion.真核生物蛋白分泌的非常规机制。
Curr Biol. 2018 Apr 23;28(8):R406-R410. doi: 10.1016/j.cub.2017.11.074.