环状ATP2A2通过上调肌球蛋白重链9促进骨肉瘤进展。
circATP2A2 promotes osteosarcoma progression by upregulating MYH9.
作者信息
Cao Xin, Meng Xianfeng, Fu Peng, Wu Lin, Yang Zhen, Chen Huijin
机构信息
Department of Trauma and Orthopaedics, Shengli Oilfield Central Hospital, Dongying, Shandong, China.
Department of Clinical Laboratory, Shengli Oilfield Central Hospital, No. 31, Jinan Road, Dongying, 257000, Shandong, China.
出版信息
Open Med (Wars). 2021 Nov 22;16(1):1749-1761. doi: 10.1515/med-2021-0370. eCollection 2021.
Osteosarcoma (OS) is a highly metastatic primary malignant tumor. CircRNA hsa_circ_0028173 (circATP2A2) has been uncovered to be related to the advancement of OS. However, the biological role of circATP2A2 in OS has not been validated. circATP2A2 and MYH9 were upregulated while miR-335-5p was downregulated in OS. OS patients with high circATP2A2 expression displayed a shorter overall survival and the area under curve of circATP2A2 was 0.77, manifesting that circATP2A2 might be a diagnostic and prognostic biomarker. circATP2A2 silencing repressed OS cell proliferation and glycolysis and constrained OS cell proliferation, glycolysis, migration, and invasion . circATP2A2 regulated MYH9 expression through sponging miR-335-5p. MiR-335-5p inhibitor reversed the repressive effect of circATP2A2 knockdown on OS cell malignancy and glycolysis. MYH9 overexpression overturned miR-335-5p upregulation-mediated OS cell malignancy and glycolysis. circATP2A2 accelerated OS cell malignancy and glycolysis through upregulating MYH9 via sponging miR-335-5p, offering a promising target for OS treatment.
骨肉瘤（OS）是一种具有高度转移性的原发性恶性肿瘤。环状RNA hsa_circ_0028173（circATP2A2）已被发现与骨肉瘤的进展有关。然而，circATP2A2在骨肉瘤中的生物学作用尚未得到验证。在骨肉瘤中，circATP2A2和肌球蛋白重链9（MYH9）上调，而微小RNA-335-5p（miR-335-5p）下调。circATP2A2表达高的骨肉瘤患者总生存期较短，circATP2A2的曲线下面积为0.77，表明circATP2A2可能是一种诊断和预后生物标志物。circATP2A2沉默可抑制骨肉瘤细胞增殖和糖酵解，并抑制骨肉瘤细胞增殖、糖酵解、迁移和侵袭。circATP2A2通过吸附miR-335-5p来调节MYH9的表达。miR-335-5p抑制剂可逆转circATP2A2敲低对骨肉瘤细胞恶性程度和糖酵解的抑制作用。MYH9过表达可逆转miR-335-5p上调介导的骨肉瘤细胞恶性程度和糖酵解。circATP2A2通过吸附miR-335-5p上调MYH9，从而加速骨肉瘤细胞的恶性程度和糖酵解，为骨肉瘤治疗提供了一个有前景的靶点。
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