Oral Diagnosis Department, Piracicaba Dental School, State University of Campinas (UNICAMP), Piracicaba, Brazil.
Pathology Department, School of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil.
Oral Dis. 2023 Apr;29(3):1017-1027. doi: 10.1111/odi.14102. Epub 2022 Jan 23.
To analyze the proteomic profile of salivary pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA) samples and correlate them with the malignant transformation of the PA.
Thirty samples (10 PA, 16 CXPA, and 4 residual PA) were microdissected and submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS). The proteomic data and protein identification were analyzed through LC-MS/MS spectra using the MaxQuant software.
The proteomic analysis identified and quantified a total of 240 proteins in which 135 were found in PA, residual PA, and CXPA. The shared proteins were divided into six subgroups, and the proteins that showed statistically significant differences (p > 0.05) and fold-change > or <2.5 in one subgroup to another subgroup were included. Seven proteins (Apolipoprotein A-I-APOA1, haptoglobin-HP, protein of the synaptonemal complex 1-SYCP1, anion transport protein of band 3-SLC4A1, subunit μ1 of AP-1 complex-AP1M1, beta subunit of hemoglobin-HBB, and dermcidin-DCD) were classified as potential protein signatures, being HP, AP1M1, and HBB with higher abundance for PA to residual PA, APOA1 with higher abundance for PA to CXPA, SLC4A1 with lower abundance in the PA to CXPA, SYCP1with lower abundance for residual PA to CXPA, and DCD with higher abundance in the CXPA with epithelial differentiation to myoepithelial differentiation.
In this work, we demonstrated the comparative proteomic profiling of PA, residual PA, and CXPA, and seven were proposed as protein signatures, some of which may be associated with the malignant phenotype acquisition.
分析唾液多形性腺瘤（PA）和癌在多形性腺瘤（CXPA）样本中的蛋白质组谱，并将其与 PA 的恶性转化相关联。
对 30 个样本（10 个 PA、16 个 CXPA 和 4 个残留 PA）进行显微切割，并进行液相色谱-串联质谱（LC-MS/MS）分析。使用 MaxQuant 软件对 LC-MS/MS 谱进行分析，对蛋白质组学数据和蛋白质鉴定进行分析。
蛋白质组学分析共鉴定和定量了 240 种蛋白质，其中 135 种在 PA、残留 PA 和 CXPA 中存在。共享蛋白质被分为六个亚组，在一个亚组到另一个亚组中，具有统计学意义差异（p>0.05）和倍数变化>或<2.5 的蛋白质被包括在内。有 7 种蛋白质（载脂蛋白 A-I-APOA1、触珠蛋白-HP、联会复合体 1 蛋白-SYCP1、带 3 阴离子转运蛋白-SLC4A1、AP-1 复合物μ1 亚基-AP1M1、血红蛋白-β亚基-HBB 和皮肤素-DCD）被归类为潜在的蛋白质特征，HP、AP1M1 和 HBB 在 PA 到残留 PA 中丰度较高，APOA1 在 PA 到 CXPA 中丰度较高，SLC4A1 在 PA 到 CXPA 中丰度较低，SYCP1 在残留 PA 到 CXPA 中丰度较低，DCD 在具有上皮分化的 CXPA 中丰度较高，向肌上皮分化。
在这项工作中，我们展示了 PA、残留 PA 和 CXPA 的比较蛋白质组学分析，并提出了 7 种作为蛋白质特征，其中一些可能与恶性表型的获得有关。
