Mejillano M R, Himes R H
Biochem Biophys Res Commun. 1986 Sep 30;139(3):967-73. doi: 10.1016/s0006-291x(86)80272-8.
In a recent study, we have shown that N10-formyltetrahydrofolate synthetase prefers (Sp)-MgATP beta S over the Rp isomer in the forward reaction. In this report the stereochemistry of ATP beta S produced from prochiral ADP beta S in the reverse reaction was determined. The ATP beta S product was purified and tested as a substrate for hexokinase (preference for the Rp isomer), adenylate kinase (preference for the Sp isomer) and N10-formyltetrahydrofolate synthetase. A comparison of kinetic constants for the product and the authentic Sp and Rp isomers shows that the product is the Sp diastereomer. 31P NMR was also used to identify the product as (Sp)-ATP beta S.
在最近的一项研究中，我们已经表明，在正向反应中，N10-甲酰四氢叶酸合成酶更喜欢（Sp）-MgATPβS而非Rp异构体。在本报告中，测定了在逆向反应中由前手性ADPβS产生的ATPβS的立体化学。纯化了ATPβS产物，并将其作为己糖激酶（偏好Rp异构体）、腺苷酸激酶（偏好Sp异构体）和N10-甲酰四氢叶酸合成酶的底物进行测试。对产物与真实的Sp和Rp异构体的动力学常数进行比较表明，产物是Sp非对映体。31P NMR也被用于鉴定产物为（Sp）-ATPβS。
