Grover Aseem, Sinha Reshma, Jyoti Divya, Faggio Caterina
School of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Bajhol, India.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Italy.
Microsc Res Tech. 2022 May;85(5):1976-1989. doi: 10.1002/jemt.24029. Epub 2021 Dec 13.
Electron microscope (EM) was developed in 1931 and since then microscopical examination of both the biological and non-biological samples has been revolutionized. Modifications in electron microscopy techniques, such as scanning EM and transmission EM, have widened their applicability in the various sectors such as understanding of drug toxicity, development of mechanism, criminal site investigation, and characterization of the nano-molecule. The present review summarizes its role in important aspects such as toxicity assessment and disease diagnosis in special reference to SARS-COV2. In the biological system, EM studies have elucidated the impact of toxicants at the ultra-structural level in various tissue in conformity to physiological alterations. Thus, EM can be concluded as an important tool in toxicity assessment and disease prognosis.
电子显微镜（EM）于1931年问世，自那时起，对生物和非生物样本的显微镜检查发生了革命性变化。电子显微镜技术的改进，如扫描电子显微镜和透射电子显微镜，扩大了它们在各个领域的适用性，如了解药物毒性、作用机制的研究、犯罪现场调查以及纳米分子的表征。本综述总结了其在毒性评估和疾病诊断等重要方面的作用，特别提及了严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。在生物系统中，电子显微镜研究已经阐明了毒物在超微结构水平上对各种组织的影响，这与生理变化相一致。因此，可以得出结论，电子显微镜是毒性评估和疾病预后的重要工具。
