Maki H, Saito M, Kobayashi T, Kawai K, Akaboshi M
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Nov;50(5):795-809. doi: 10.1080/09553008614551231.
The effects on the cellular viability and induction and repair kinetics of DNA strand breaks in HeLa cells were examined after exposure to a thermal neutron beam and compared with those after gamma-irradiation. The thermal neutron survival curve had no initial shoulder. The relative biological effectiveness (r.b.e.) value of the neutron beam was determined to be 2.2 for cell killing (ratio of D0 values), 1.8 and 0.89 for single strand breakage (ssb) by alkaline sedimentation and alkaline elution respectively, and for double strand breakage (dsb) 2.6 by neutral elution. No difference was observed between thermal neutrons and gamma-rays in the repair kinetics of ssb and dsb. It is suggested that the effect induced by the intracellular nuclear reaction, 14N(n,p)14C is mainly responsible for the high r.b.e. values observed.
在将HeLa细胞暴露于热中子束后，检测了其对细胞活力以及DNA链断裂的诱导和修复动力学的影响，并与γ射线照射后的情况进行了比较。热中子存活曲线没有初始坪区。确定中子束的相对生物效应（r.b.e.）值对于细胞杀伤（D0值之比）为2.2，对于通过碱性沉降和碱性洗脱的单链断裂（ssb）分别为1.8和0.89，对于通过中性洗脱的双链断裂（dsb）为2.6。在ssb和dsb的修复动力学方面，未观察到热中子和γ射线之间存在差异。有人提出，细胞内核反应14N(n,p)14C所诱导的效应是观察到高r.b.e.值的主要原因。
