Berkman E
Mikrobiyol Bul. 1986 Apr;20(2):76-83.
Present study is carried out in order to determine the incidence of Haemophilus influenzae in the throat cultures examined Microbiology Laboratory of Hacettepe Children's Hospital. Throat culture specimens were first inoculated into blood agar and also tryptic soy agar plates. BVX, BV and BX discs were placed on inoculated tryptic soy agar surface for selective growth. The colonies grown around discs were also subjected to agglutination test with specific monovalent and polyvalent antisera for definite diagnosis. As a result, 37 H. influenzae (% 2.96) isolated out of 1249 throat culture tested and 12 (0.96) of isolates classified as type b.
本研究旨在确定在哈杰泰佩儿童医院微生物实验室检查的咽喉培养物中流感嗜血杆菌的发生率。咽喉培养标本首先接种到血琼脂和胰蛋白胨大豆琼脂平板上。将BVX、BV和BX圆盘放置在接种的胰蛋白胨大豆琼脂表面以进行选择性生长。圆盘周围生长的菌落也用特异性单价和多价抗血清进行凝集试验以进行明确诊断。结果，在1249份检测的咽喉培养物中分离出37株流感嗜血杆菌（2.96%），其中12株（0.96%）被归类为b型。
