Trutin-Ostović C, Golubić M, Matović M, Marusić M
Cancer Immunol Immunother. 1986;23(2):130-6. doi: 10.1007/BF00199819.
BALB/c mice were treated s.c. with 3-methylcholanthrene (MCA), and tumor incidence and growth were followed for 9 months. Immunological status of mice was altered by various treatments. Thymectomized, lethally irradiated, bone marrow reconstituted mice served as T-cell deficient recipients. In order to suppress natural killer (NK)-cell/macrophage functions some mice were injected with silica particles; to enhance these functions some mice were given Corynebacterium parvum (CP). Silica and CP were given simultaneously with MCA to test their influence on the presumed function of surveillance of tumor incidence, and also 2 months after MCA to test their influence on the growth of greater numbers of transformed host cells. Almost all mice developed tumors at the inoculation site and at the end of the observation period there was no difference in tumor incidence among 9 experimental groups. However, in T-cell deficient mice we observed shorter tumor duration and earlier death than in normal mice. Silica particles appeared to enhance tumor growth but the differences compared to normal controls were not significant. A single injection of CP simultaneously with MCA caused earlier tumor appearance but also slowed its growth. In contrast, CP given 2 months after MCA significantly delayed the appearance of the tumors. In regard to the tumor growth immunosuppression had stronger effects in males than in females; the opposite was true for immunostimulation treatments. We concluded that immunological status does not influence long-term tumor incidence, but that both T-cell and NK-cell/macrophage compartments strongly influence the parameters of growth of chemically induced tumors, i.e., the immune and natural resistance mechanisms do not influence the frequency of de novo arising tumors but both can slow down tumor growth.
将BALB/c小鼠皮下注射3-甲基胆蒽（MCA），并跟踪肿瘤发生率和生长情况9个月。通过各种处理改变小鼠的免疫状态。胸腺切除、接受致死剂量照射、骨髓重建的小鼠作为T细胞缺陷受体。为了抑制自然杀伤（NK）细胞/巨噬细胞功能，一些小鼠注射二氧化硅颗粒；为了增强这些功能，一些小鼠给予短小棒状杆菌（CP）。二氧化硅和CP与MCA同时给药，以测试它们对假定的肿瘤发生率监测功能的影响，并且在MCA给药2个月后给药，以测试它们对大量转化宿主细胞生长的影响。几乎所有小鼠在接种部位都发生了肿瘤，在观察期结束时，9个实验组的肿瘤发生率没有差异。然而，我们观察到T细胞缺陷小鼠的肿瘤持续时间比正常小鼠短，死亡更早。二氧化硅颗粒似乎促进了肿瘤生长，但与正常对照组相比差异不显著。与MCA同时单次注射CP会导致肿瘤出现更早，但也会减缓其生长。相比之下，MCA给药2个月后给予CP会显著延迟肿瘤的出现。关于肿瘤生长，免疫抑制在雄性小鼠中比在雌性小鼠中作用更强；免疫刺激处理则相反。我们得出结论，免疫状态不影响长期肿瘤发生率，但T细胞和NK细胞/巨噬细胞区室都强烈影响化学诱导肿瘤的生长参数，即免疫和天然抵抗机制不影响新生肿瘤的频率，但两者都可以减缓肿瘤生长。
