Diegelmann R F, Schuller-Levis G, Cohen I K, Kaplan A M
Clin Immunol Immunopathol. 1986 Dec;41(3):331-41. doi: 10.1016/0090-1229(86)90004-8.
Fibroblasts migrate in vitro in response to a variety of chemoattractants including cellular, humoral, and connective tissue components. This report describes a low molecular weight, macrophage-derived chemotactic substance for fibroblasts (MDCF-F). Thioglycolate-elicited peritoneal exudate cells were collected from mice 4 days postinjection. Platelets were removed and glass-adherent macrophages were selected in the absence of fetal calf serum. The macrophage culture medium was then tested for chemotactic activity for 3T3 mouse fibroblasts using a modified Boyden chamber. Chemotactic activity was observed in the macrophage medium after a 48-hr culture period and increased during the next 24 hr. The activity was sensitive to heat treatment at 56 degrees C for 30 min, could be effectively absorbed out of the culture medium by preincubation with 3T3 fibroblasts, and was not recognized by human fibroblasts. Molecular-sieve chromatography indicated a molecular weight below 10,000. This low molecular weight, macrophage-derived chemoattractant for fibroblasts may represent a rapidly diffusable substance involved in the recruitment of fibroblasts to sites of inflammation.
成纤维细胞在体外会对多种趋化因子产生迁移反应，这些趋化因子包括细胞、体液和结缔组织成分。本报告描述了一种低分子量的、巨噬细胞衍生的成纤维细胞趋化物质（MDCF-F）。在注射硫代乙醇酸盐4天后从小鼠收集腹腔渗出细胞。去除血小板，并在无胎牛血清的情况下选择玻璃黏附巨噬细胞。然后使用改良的博伊登小室检测巨噬细胞培养基对3T3小鼠成纤维细胞的趋化活性。在培养48小时后在巨噬细胞培养基中观察到趋化活性，并在接下来的24小时内增加。该活性对56℃热处理30分钟敏感，通过与3T3成纤维细胞预孵育可有效从培养基中吸收，且人成纤维细胞无法识别。分子筛色谱显示分子量低于10000。这种低分子量的、巨噬细胞衍生的成纤维细胞趋化因子可能代表一种参与将成纤维细胞募集到炎症部位的快速扩散物质。
