乳酸菌属对γ射线照射大鼠睾丸细胞凋亡相关基因 BCL2、BAX 和 caspase 3 的影响。
Effect of Lactobacillus species on apoptosis-related genes BCL2, BAX, and caspase 3 in the testes of gamma-irradiated rats.
机构信息
Tehran University of Medical Sciences, Allied Medical Sciences School, Technology of Radiology and Radiotherapy Department - Tehran, Iran.
Behbahan Faculty of Medical Sciences, Radiology Technology Department - Behbahan, Iran.
出版信息
Rev Assoc Med Bras (1992). 2021 Nov;67(11):1581-1585. doi: 10.1590/1806-9282.20210634.
OBJECTIVE
Ionizing radiation has various applications, including uses in medicine, industry, agriculture, and research. However, ionizing radiation is accompanied by side effects in normal radiosensitive tissues. Probiotics as natural radioprotective agents can protect normal tissues from ionizing radiation. In this regard, this study aimed to investigate the effect of Lactobacillus species on apoptosis-related genes BCL2, BAX, and caspase 3 (CASP3) in the testes of gamma-irradiated rats.
METHODS
A total of 30 male Wistar rats were involved in this study. The animals received the whole- body radiation with the dose rate of 2 Gy gamma-ray and were orally gavaged with 0.2 mL of 1×1010 Lactobacillus species in phosphate-buffered saline (PBS) for 4 weeks. Then, the relative gene expression levels of BCL2, BAX, and CASP3 in the testis were assessed by using the quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
Compared with the control group, radiation significantly downregulated the BCL2 and upregulated the BAX and CASP3 genes (p<0.0001). However, Lactobacillus species significantly reversed these effects.
CONCLUSION
All in all, according to our results, employing Lactobacilli probiotics as a natural radioprotector may protect radiosensitive tissue from damage.
目的
电离辐射有多种应用，包括在医学、工业、农业和研究中的应用。然而，电离辐射伴随着正常放射敏感组织的副作用。益生菌作为天然辐射防护剂，可以保护正常组织免受电离辐射的伤害。在这方面，本研究旨在探讨乳杆菌属对γ 射线照射大鼠睾丸中凋亡相关基因 BCL2、BAX 和半胱氨酸天冬氨酸蛋白酶 3（CASP3）的影响。
方法
本研究共涉及 30 只雄性 Wistar 大鼠。动物接受 2Gy γ 射线全身照射，并用磷酸盐缓冲液（PBS）中的 0.2ml1×1010 乳杆菌属口服灌胃 4 周。然后，通过实时定量聚合酶链反应（qRT-PCR）评估睾丸中 BCL2、BAX 和 CASP3 基因的相对表达水平。
结果
与对照组相比，辐射显著下调了 BCL2 并上调了 BAX 和 CASP3 基因（p<0.0001）。然而，乳杆菌属显著逆转了这些效应。
结论
总之，根据我们的结果，使用乳杆菌属益生菌作为天然辐射防护剂可能会保护放射敏感组织免受损伤。
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