Di Mario U, Pietravalle P, Napoli A, Morano S, Mancuso M, Gambardella S, Andreani D
Horm Metab Res. 1986 Oct;18(10):689-92. doi: 10.1055/s-2007-1012408.
The detection of microalbuminuria has a high prognostic value in diabetic patients with no symptoms of clinical nephropathy. Attention has been focused on the development of a simple, reproducible, specific, and, above all, sensitive method to detect albuminuria in the urine. The method is based on the competitive binding between albumin in the urine to be tested and a fixed amount of radiolabelled albumin to anti-albumin immunoglobulins in solid phase. The immunoglobulin C fraction of a rabbit anti-human albumin antiserum was left to coat highly adsorbent polystyrene microtitre tubes. While the aspecific tube binding was overcome by saturation with a solution of gelatine, increasing dilutions of standard albumin or the diluted urine samples to be tested were left to incubate with an equal volume of radiolabelled albumin at room temperature for 1 hour. The cold/hot albumin mixture was added to anti-albumin immunoglobulin-coated tubes, which had been repeatedly washed, and left to incubate. After washing, radioactivity was assessed. This assay has proved to be fast, simple and highly sensitive since it detects up to 25 ng of albumin per ml and is of value in large-scale screening for microalbuminuria in diabetic patients.
在无临床肾病症状的糖尿病患者中，微量白蛋白尿的检测具有很高的预后价值。人们一直致力于开发一种简单、可重复、特异且最重要的是灵敏的方法来检测尿中的白蛋白尿。该方法基于待测尿液中的白蛋白与固定量的放射性标记白蛋白在固相上与抗白蛋白免疫球蛋白之间的竞争性结合。用兔抗人白蛋白抗血清的免疫球蛋白C组分包被高吸附性聚苯乙烯微量滴定管。用明胶溶液饱和可克服非特异性管结合，将标准白蛋白的稀释液或待测尿液样本的稀释液与等体积的放射性标记白蛋白在室温下孵育1小时。将冷/热白蛋白混合物加入已反复洗涤的抗白蛋白免疫球蛋白包被管中并孵育。洗涤后，评估放射性。该检测方法已被证明快速、简单且高度灵敏，因为它每毫升可检测到高达25纳克的白蛋白，对糖尿病患者微量白蛋白尿的大规模筛查具有重要价值。
