Gaevskaia V A, Azhitskiĭ G Iu
Ukr Biokhim Zh (1978). 1978 Nov-Dec;50(6):735-8.
The article deals with the isoelectric spectrum of human serum albumin isolated by electrophoresis in polyacrylamide gel, on agar-agar, by salting out with ammonium sulphate and also by the Cohn method. It is shown that the way of albumin isolation, except the Cohn method, does not affect the quantitative and qualitative characteristics of its isoelectric spectrum obtained on ampholines by focusing. Fractions isolated from healthy people have always pI 4.7, 5.1, 5.5, their ratio being different. Albumin and its fractions are identical immunochemically. Dispersion of the fraction content optic rotation, except the fraction with pI 5,1, is the same as in initial albumin. Bilirubin is bound mainly (80%) by the fraction with pI 4.7 and it is absent in the fraction with pI 5.5.
本文研究了通过聚丙烯酰胺凝胶电泳、琼脂电泳、硫酸铵盐析以及科恩方法分离得到的人血清白蛋白的等电谱。结果表明，除科恩方法外，白蛋白的分离方式不会影响通过两性电解质聚焦法获得的等电谱的定量和定性特征。从健康人分离出的组分的等电点始终为4.7、5.1、5.5，其比例各不相同。白蛋白及其组分在免疫化学上是相同的。除了等电点为5.1的组分外，各组分含量的旋光度分散情况与初始白蛋白相同。胆红素主要（80%）与等电点为4.7的组分结合，而等电点为5.5的组分中不存在胆红素。
