Sopkina D A, Ostolovskiĭ E M, Ivlev V N
Ukr Biokhim Zh (1978). 1978 Nov-Dec;50(6):730-4.
The isoelectric spectrum of albumin isolated from the liver and blood serum of 30-, 45- and 90-day rabbits was studied. By the method of isoelectric focusing in the boron-borate buffer--mannitol system. It is shown that the liver albumin displays heterogeneity ans is separated into four-five fractions, with pJ 4.8-6.0. Age peculiarities are found for the isoelectric spectrum of this protein. The serum albumin spectrum for rabbits of the studied age groups is characterized by the presence of a homogeneous peak with pJ 5.59, 5.57 and 5.47 corresponding to 30, 45 and 90-day age, respectively. Identity of serum albumin and protein of some liver albumin spectrum components is established by analyzing the pattern of the isoelectric spectrum for a mixture of preparations of 90-day rabbit proteins under comparison.
研究了从30日龄、45日龄和90日龄兔子的肝脏和血清中分离出的白蛋白的等电谱。采用硼酸盐缓冲液-甘露醇体系中的等电聚焦法。结果表明，肝脏白蛋白具有异质性，可分为4-5个组分，其等电点为4.8-6.0。发现该蛋白质的等电谱存在年龄特异性。所研究年龄组兔子的血清白蛋白谱的特征是分别存在一个等电点为5.59、5.57和5.47的单一峰，对应于30日龄、45日龄和90日龄。通过分析90日龄兔子蛋白质制剂混合物的等电谱模式，确定了血清白蛋白与某些肝脏白蛋白谱组分的蛋白质的同一性。
