Zhou Ying, Zhang Yumo, Zhang Xiaoyan, Chen Zhuo, Dong Jian, Yang Chao, Lv Taotao, Yu Tianyuan, Lu Mengqian
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
Department of Traditional Chinese Medicine, Aerospace Center Hospital, College of Aerospace Clinical Medicine of Peking University, Beijing 100049, China.
Evid Based Complement Alternat Med. 2021 Dec 6;2021:6897124. doi: 10.1155/2021/6897124. eCollection 2021.
To study the effects of five manipulations in rats with deep vein thrombosis (DVT) and to explore how to safely perform in the treatment of thrombotic diseases.
Seventy-two male Sprague-Dawley (SD) rats were randomly divided into the model, pointing manipulation, plucking manipulation, kneading manipulation, pushing manipulation, and pulling manipulation groups ( = 12). DVT model was established by incomplete ligation. The intervention was started on the next day after modeling and applied once a day 10 times by the manipulation simulators. On the 3 and 10 days after intervention, respectively, the effects of on thrombosis were evaluated based on thrombus elasticity, blood coagulation, fibrinolytic function and blood rheology with the ultrasound elastography, four coagulation tests, enzyme linked immunosorbent assay (ELISA), and hemorheology tests.
In the pointing manipulation group, the strain rate ratio, 6-ketoprostaglandin F1 (6-Keto-PGF1), and high shear rate were decreased, and the thromboxane B (TXB) content was increased ( < 0.05). In the plucking manipulation group, the D-dimer and 6-Keto-PGF1 contents were increased, prothrombin time (PT) was shortened, and activated partial thromboplastin time (APTT) was activated, and the high shear rate and plasma viscosity were decreased ( < 0.05). In the kneading manipulation group, APTT was shortened, and 6-Keto-PGF1, high shear rate, and plasma viscosity were decreased ( < 0.05). In the pushing manipulation group, the strain rate ratio, low shear rate, and high shear rate were all decreased ( < 0.05). In the pulling manipulation group, both the strain rate ratio and the low shear rate were decreased ( < 0.05). The 6-Keto-PGF1 changes on the 3 and 10 days after intervention were opposite in the pushing manipulation group and the pulling manipulation group ( < 0.05).
The pointing, pushing, and pulling manipulations seem to be safe in the early period of thrombosis, but the risk is likely to be elevated as the treatment course of intervention increases. The plucking and kneading manipulations potentially have certain risks in the treatment of DVT in rats.
研究五种手法对大鼠深静脉血栓形成（DVT）的影响，探讨在血栓性疾病治疗中如何安全地实施手法治疗。
将72只雄性Sprague-Dawley（SD）大鼠随机分为模型组、点穴手法组、弹拨手法组、揉按手法组、推按手法组和牵按手法组（每组 = 12只）。采用不完全结扎法建立DVT模型。造模后次日开始干预，由手法模拟装置每天进行1次，共10次。分别在干预后第3天和第10天，通过超声弹性成像、四项凝血试验、酶联免疫吸附测定（ELISA）和血液流变学检测，基于血栓弹性、凝血、纤溶功能和血液流变学评估手法对血栓形成的影响。
在点穴手法组中，应变率比值、6-酮前列腺素F1（6-Keto-PGF1）和高切变率降低，血栓素B（TXB）含量增加（P < 0.05）。在弹拨手法组中，D-二聚体和6-Keto-PGF1含量增加，凝血酶原时间（PT）缩短，活化部分凝血活酶时间（APTT）活化，高切变率和血浆黏度降低（P < 0.05）。在揉按手法组中，APTT缩短，6-Keto-PGF1、高切变率和血浆黏度降低（P < 0.05）。在推按手法组中，应变率比值、低切变率和高切变率均降低（P < 0.05）。在牵按手法组中，应变率比值和低切变率均降低（P < 0.05）。推按手法组和牵按手法组干预后第3天和第10天的6-Keto-PGF1变化相反（P < 0.05）。
点穴、推按和牵按手法在血栓形成早期似乎是安全的，但随着干预疗程的增加，风险可能会升高。弹拨和揉按手法在大鼠DVT治疗中可能存在一定风险。
