Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Methods Mol Biol. 2022;2412:457-470. doi: 10.1007/978-1-0716-1892-9_25.
The emergence of recombinant DNA technology has led to the exploration of the use of the technology to develop novel vaccines. With a fundamental role in vaccines design, several immunoinformatics tools have been created to identify isolated epitopes that stimulate a specific immune response, contributing to effective vaccines development. In the past, vaccine development projects relied entirely on animal experimentation, a relatively expensive and time-consuming process. Currently, use of immunoinformatics tools play a vital role in the antigen analysis and refinement, allowing the identification of possible protective epitopes capable of stimulating convenient humoral or cellular immune responses, in addition to facilitating time and cost reduction of vaccine production. The vaccination aimed at bacterial species of Clostridium spp. has been considered a promising example of use of these approaches in recent years. Based on the literature search, it is possible to understand the best immunoinformatics software used by researchers that facilitate recombinant vaccine antigens design and development. This chapter presents an overview of how these tools are supporting the antigen engineering, aiming at increasing the efficiency of inducing protective immune response in animals.
重组 DNA 技术的出现促使人们探索利用该技术开发新型疫苗。在疫苗设计中具有重要作用的几种免疫信息学工具被创建，以识别能够刺激特定免疫反应的分离表位，从而有助于开发有效的疫苗。过去，疫苗开发项目完全依赖于动物实验，这是一个相对昂贵和耗时的过程。目前，免疫信息学工具的使用在抗原分析和优化中发挥着重要作用，有助于识别可能具有保护性的表位，从而刺激方便的体液或细胞免疫反应，同时还能减少疫苗生产的时间和成本。近年来，针对梭菌属（Clostridium spp.）细菌的疫苗接种被认为是此类方法的一个很有前途的应用实例。通过文献检索，可以了解研究人员使用的最佳免疫信息学软件，这些软件可用于设计和开发重组疫苗抗原。本章概述了这些工具如何支持抗原工程，旨在提高在动物中诱导保护性免疫反应的效率。
