Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., Lublin 20-033, Poland.
Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., Lublin 20-033, Poland.
J Invertebr Pathol. 2022 Jan;187:107706. doi: 10.1016/j.jip.2021.107706. Epub 2021 Dec 15.
The role of Pseudomonas aeruginosa exotoxin A in the modulation of humoral immune response parameters in the hemolymph of Galleria mellonella larvae was investigated. Our results indicate that exoA can play a role of a virulence factor by inhibiting insect PO, lysozyme, and antibacterial activity and decreasing the apoLp-III protein level significantly. No peptide bands with molecular mass below 6.5 kDa were detected in the hemolymph of exoA-treated larvae. We provided evidence for involvement of exoA in the pathogenicity of P. aeruginosa against G. mellonella and the usefulness of the insect as a model for analysis of P. aeruginosa toxins.
本研究旨在探讨铜绿假单胞菌外毒素 A（Pseudomonas aeruginosa exotoxin A，exoA）在调控家蚕幼虫血淋巴体液免疫反应参数中的作用。结果表明，exoA 可通过抑制昆虫酚氧化酶（PO）、溶菌酶和抗菌活性，并显著降低载脂蛋白 Lp-III 蛋白水平，从而发挥毒力因子的作用。在 exoA 处理的幼虫血淋巴中，未检测到分子量低于 6.5 kDa 的肽带。本研究为 exoA 参与铜绿假单胞菌对家蚕的致病性提供了证据，并证明了家蚕作为分析铜绿假单胞菌毒素的模型的有用性。
