Forskolin sensitizes human platelet adenylate cyclase to modulation of substrate (MgATP) affinity by hormones.
作者信息
Watanabe Y, Jakobs K H
出版信息
Biochem J. 1986 Jul 1;237(1):273-6. doi: 10.1042/bj2370273.
Abstract
Stimulation of human platelet adenylate cyclase by the diterpene forskolin is associated with a decrease in the apparent substrate (MgATP) affinity of the enzyme. Addition of the stimulatory hormone prostaglandin E1 not only further increased the Vmax. of the forskolin-stimulated platelet adenylate cyclase but also caused a further increase in the Km value for MgATP, by up to 20-fold compared with basal conditions. On the other hand, the inhibitory hormone adrenaline decreased not only the Vmax. but also the Km value of the platelet adenylate cyclase stimulated by forskolin, with or without prostaglandin E1 present. The data indicate that forskolin sensitizes human platelet adenylate cyclase to modulation of substrate (MgATP) affinity by hormones, but there is no such effect in the absence of the diterpene.
摘要
相似文献
1
Forskolin sensitizes human platelet adenylate cyclase to modulation of substrate (MgATP) affinity by hormones.
Biochem J. 1986 Jul 1;237(1):273-6. doi: 10.1042/bj2370273.
2
The local anaesthetic benzyl alcohol attenuates the alpha 2-adrenoceptor-mediated inhibition of human platelet adenylate cyclase activity when stimulated by prostaglandin E1, but not that stimulated by forskolin.
Biochem J. 1989 Dec 1;264(2):483-8. doi: 10.1042/bj2640483.
3
Inhibition of Ns-stimulated human platelet adenylate cyclase by forskolin.
Mol Pharmacol. 1986 Mar;29(3):258-63.
4
Stimulation and inhibition of rat basophilic leukemia cell adenylate cyclase by forskolin.
Biochim Biophys Acta. 1985 Sep 30;846(3):356-63. doi: 10.1016/0167-4889(85)90006-0.
5
Relationship between forskolin and calcium-calmodulin stimulation of rat cerebral cortex adenylate cyclase: enzyme activation modulates substrate (MgATP) affinity.
Pharmacol Toxicol. 1988 Aug;63(2):90-5. doi: 10.1111/j.1600-0773.1988.tb00917.x.
6
Protein kinase C interferes with Ni-mediated inhibition of human platelet adenylate cyclase.
FEBS Lett. 1985 Nov 11;192(1):23-7. doi: 10.1016/0014-5793(85)80035-1.
7
Modulation of adenylate cyclase of human platelets by phorbol ester. Impairment of the hormone-sensitive inhibitory pathway.
Eur J Biochem. 1985 Sep 2;151(2):425-30. doi: 10.1111/j.1432-1033.1985.tb09119.x.
8
Human platelet adenylate cyclase: persistent binding of guanine nucleotide is central to the regulation of both hormone-stimulated and basal activities.
Biochim Biophys Acta. 1985 Jun 30;845(3):411-20. doi: 10.1016/0167-4889(85)90206-x.
9
Platelet prostaglandin E1 hyposensitivity in schizophrenia: reduction of prostaglandin E1- or forskolin-stimulated cyclic AMP response in platelets.
Life Sci. 1989;45(22):2135-40. doi: 10.1016/0024-3205(89)90079-9.
10
Inhibition of adenylate cyclase activity during the reversible shape change in human platelets.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):649-57. doi: 10.1016/s0006-291x(88)80088-3.
本文引用的文献
1
Regulation of adenylate cyclase of human platelet membranes by forskolin.
J Biol Chem. 1982 Jul 10;257(13):7485-90.
2
Inhibition of NS-protein-stimulated human-platelet adenylate cyclase by epinephrine and stable GTP analogs.
Eur J Biochem. 1983 Aug 1;134(2):351-4. doi: 10.1111/j.1432-1033.1983.tb07574.x.
3
Interactions of forskolin and adenylate cyclase. Effects on substrate kinetics and protection against inactivation by heat and N-ethylmaleimide.
J Biol Chem. 1983 Mar 10;258(5):2960-5.
4
Hormonal inhibition of adenylate cyclase. A crucial role for Mg2+.
Mol Pharmacol. 1984 Sep;26(2):180-6.
5
Forskolin, adenylate cyclase, and cell physiology: an overview.
Adv Cyclic Nucleotide Protein Phosphorylation Res. 1984;17:81-9.
6
G proteins and dual control of adenylate cyclase.
Cell. 1984 Mar;36(3):577-9. doi: 10.1016/0092-8674(84)90336-2.
7
Hormone receptor modulates the regulatory component of adenylyl cyclase by reducing its requirement for Mg2+ and enhancing its extent of activation by guanine nucleotides.
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5179-83. doi: 10.1073/pnas.79.17.5179.
8
Forskolin: a unique diterpene activator of cyclic AMP-generating systems.
J Cyclic Nucleotide Res. 1981;7(4):201-24.
9
Uncoupling of alpha-adrenoceptor-mediated inhibition of human platelet adenylate cyclase by N-ethylmaleimide.
J Biol Chem. 1982 Mar 25;257(6):2829-33.
10
Adenylate cyclase: the role of magnesium and other divalent cations.
Mol Cell Biochem. 1980 Dec 10;33(1-2):67-92. doi: 10.1007/BF00224572.
Zotero 插件