Rennick D, Yang G, Gemmell L, Lee F
Blood. 1987 Feb;69(2):682-91.
A stromal cell line, GY30, was cloned from mouse bone marrow adherent cell layers. In culture, GY30 cells sustain the production of granulocyte-macrophage progenitor cells (GM-CFU) but fail to support the survival of pluripotential stem cells (CFU-S). GY30 cells secrete two growth factor activities distinct from interleukin-3 (IL-3), IL-2, and macrophage colony-stimulating factor (M-CSF) but functionally similar to GM-CSF and G-CSF. The production of both CSFs is increased 70- to 200-fold by treating GY30 cells with lipopolysaccharide or IL-1. RNA blot analysis reveals the presence of GM-CSF and G-CSF transcripts and demonstrates that IL-1 regulates the production of both factors at the mRNA level. Further, these studies show that the GM-CSF secreted by GY30 cells is structurally similar to the GM-CSF produced by activated T cells.
一种基质细胞系GY30是从小鼠骨髓贴壁细胞层克隆而来的。在培养过程中，GY30细胞能持续产生粒细胞-巨噬细胞祖细胞（GM-CFU），但无法支持多能干细胞（CFU-S）的存活。GY30细胞分泌两种与白细胞介素-3（IL-3）、IL-2和巨噬细胞集落刺激因子（M-CSF）不同的生长因子活性物质，但在功能上与GM-CSF和G-CSF相似。用脂多糖或IL-1处理GY30细胞后，这两种集落刺激因子的产量会增加70至200倍。RNA印迹分析揭示了GM-CSF和G-CSF转录本的存在，并表明IL-1在mRNA水平上调节这两种因子的产生。此外，这些研究表明，GY30细胞分泌的GM-CSF在结构上与活化T细胞产生的GM-CSF相似。
