Indrová M, Bubeník J, Toulcová A
Folia Biol (Praha). 1986;32(6):392-8.
Highly purified human recombinant interleukin 2 induced cytotoxicity in mouse spleen cells against mouse sarcoma cells when added during the 51Cr microcytotoxicity assay. It elicited similar levels of killer cell activation as did human lymphoid (Jurkat leukaemia-derived) or mouse lymphoid (EL-4 leukaemia-derived) IL-2 preparations. The susceptibility of six MC-induced mouse sarcomas to the cytolytic effect of lymphokine-activated killer cells was compared. Five (MC11, MC13, MC14, MC15, MC16) of six mouse sarcoma cell lines examined were sensitive in vitro to the LAK cell effect, whereas one cell line (MC12) was resistant. Since the sensitive and resistant target cell lines had been induced with the same carcinogen and in mice of the same genotype, they represent a very useful model for investigation of target cell structures responsible for the sensitivity to the LAK cell effect.
在51Cr微量细胞毒性测定过程中添加高度纯化的人重组白细胞介素2时，它可诱导小鼠脾细胞对小鼠肉瘤细胞产生细胞毒性。它引发的杀伤细胞激活水平与人类淋巴细胞（源自Jurkat白血病）或小鼠淋巴细胞（源自EL-4白血病）的白细胞介素2制剂相似。比较了六种甲基胆蒽诱导的小鼠肉瘤对淋巴因子激活的杀伤细胞的细胞溶解作用的敏感性。在所检测的六种小鼠肉瘤细胞系中，有五种（MC11、MC13、MC14、MC15、MC16）在体外对LAK细胞作用敏感，而一种细胞系（MC12）具有抗性。由于敏感和抗性靶细胞系是用相同致癌物在相同基因型的小鼠中诱导产生的，它们代表了一个非常有用的模型，用于研究负责对LAK细胞作用敏感性的靶细胞结构。
