成人及脐带动静脉培养的人血管内皮细胞中的血管性血友病因子

von Willebrand factor in cultured human vascular endothelial cells from adult and umbilical cord arteries and veins.

作者信息

van Wachem P B, Reinders J H, van Buul-Wortelboer M F, de Groot P G, van Aken W G, van Mourik J A

出版信息

Thromb Haemost. 1986 Oct 21;56(2):189-92.

Abstract

Endothelial cells were cultured from various human arteries and veins, obtained from adult individuals and from umbilical cords. We compared the storage and secretion of von Willebrand factor by endothelial cells from umbilical veins with that of endothelial cells cultured from a number of adult vessels, including aorta, arteria iliaca, vena saphena magna and vena cava. There were no differences in the way the cultured endothelial cells handled the von Willebrand factor they synthesized. Endothelial cells from the various vessels responded to stimuli in secreting stored von Willebrand factor. The cells also responded to thrombin and ionophore A23187 in producing enhanced amounts of prostacyclin. Thus, cultured umbilical vein endothelial cells have properties that are very similar to those of cultured endothelial cells of various other origins. It is concluded that foetal venous cells provide a representative model for studies of endothelial cell von Willebrand factor biosynthesis and prostacyclin production.

摘要

内皮细胞取自成年个体和脐带的各种人体动脉和静脉并进行培养。我们比较了脐静脉内皮细胞与从包括主动脉、髂动脉、大隐静脉和腔静脉在内的一些成年血管培养的内皮细胞中血管性血友病因子的储存和分泌情况。培养的内皮细胞处理其合成的血管性血友病因子的方式没有差异。来自各种血管的内皮细胞在分泌储存的血管性血友病因子时对刺激有反应。这些细胞在产生更多前列环素方面也对凝血酶和离子载体A23187有反应。因此，培养的脐静脉内皮细胞具有与来自其他各种来源的培养内皮细胞非常相似的特性。得出的结论是，胎儿静脉细胞为研究内皮细胞血管性血友病因子生物合成和前列环素产生提供了一个代表性模型。