State Key Laboratory of Food Science and Technology, Institute of Nutrition and School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
J Agric Food Chem. 2021 Dec 29;69(51):15575-15584. doi: 10.1021/acs.jafc.1c05830. Epub 2021 Dec 20.
The intestinal barrier is essential for maintaining human intestinal health. The growing number of studies has shown that both puerarin and tryptophan and its metabolites have a beneficial effect on the intestinal barrier. This study aims at the combination of puerarin and tryptophan or its metabolites for improving the intestinal barrier. In our study, 40 female Sprague-Dawley rats were randomly divided into five groups ( = 8) for a 4-week experiment and dextran sodium sulfate was used to induce an intestinal barrier injury in rats. Our results showed that puerarin combined with tryptophan or its metabolites (indole-3-propionic acid, IPA) improved the intestinal barrier by enhancing the mucus layer barrier, which was mainly achieved by increasing the number of goblet cells and promoting the secretion of MUC2. Both TRPM5 and VAMP8 promoted MUC2 secretion in goblet cells through exocytosis, but their mechanisms of action are different. In our study, we found that puerarin and tryptophan showed different effects on TRPM5 and VAMP8, respectively. Puerarin enhances the expression of TRPM5, and tryptophan inhibits the expression of TRPM5; however, puerarin and tryptophan have no significant effect on the expression of VAMP8.
肠道屏障对于维持人体肠道健康至关重要。越来越多的研究表明，葛根素和色氨酸及其代谢物对肠道屏障都具有有益作用。本研究旨在探讨葛根素与色氨酸或其代谢物联合应用对改善肠道屏障的作用。本研究将 40 只雌性 Sprague-Dawley 大鼠随机分为五组（每组 8 只）进行为期 4 周的实验，采用葡聚糖硫酸钠诱导大鼠肠道屏障损伤。结果表明，葛根素与色氨酸或其代谢物（吲哚-3-丙酸，IPA）联合使用可通过增强黏液层屏障来改善肠道屏障，主要通过增加杯状细胞数量和促进 MUC2 的分泌来实现。TRPM5 和 VAMP8 均可通过胞吐作用促进杯状细胞中 MUC2 的分泌，但作用机制不同。本研究发现，葛根素和色氨酸对 TRPM5 和 VAMP8 表现出不同的作用。葛根素增强了 TRPM5 的表达，而色氨酸抑制了 TRPM5 的表达；然而，葛根素和色氨酸对 VAMP8 的表达没有显著影响。
