Department of Food and Life Science, School of Life and Environmental Science, Azabu University, Sagamihara, Japan.
Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
Genes Cells. 2022 Feb;27(2):138-144. doi: 10.1111/gtc.12915. Epub 2022 Jan 5.
Skeletal muscle atrophy is the loss of muscle tissue caused by factors such as inactivity, malnutrition, aging, and injury. In this study, we aimed to investigate whether egg components exert inhibitory effects on muscle atrophy. An egg mix solution was orally administered for 10 consecutive days to male C57BL/6J mice injected with cardiotoxin in the tibialis anterior (TA) muscle. The administration of egg mixture significantly decreased the atrogin-1 and MuRF-1 protein levels, key factors in muscle atrophy, as observed by western blotting. Furthermore, we investigated the effects of egg components such as avidin, lecithin, biotin, 3-sn-phosphatidylcholine, and L-α-phosphatidylcholine on dexamethasone (DEX)-treated C2C12 myotubes. Lecithin, biotin, 3-sn-phosphatidylcholine, and L-α-phosphatidylcholine in egg yolk significantly recovered the diameters of C2C12 myotubes decreased upon DEX application. Avidin did not show such reversal. Biotin, 3-sn-phosphatidylcholine, and L-α-phosphatidylcholine also attenuated atrogin-1 protein expression enhanced by DEX. Our findings reveal that egg yolk components could contribute to the reversal of skeletal muscle atrophy induced by muscle injury.
骨骼肌萎缩是由不活动、营养不良、衰老和损伤等因素引起的肌肉组织丧失。在这项研究中，我们旨在研究鸡蛋成分是否对肌肉萎缩有抑制作用。将蛋黄混合物溶液连续口服 10 天，给在前胫骨肌（TA）肌肉注射心脏毒素的雄性 C57BL/6J 小鼠。通过 Western blot 观察到，蛋黄混合物的给药显著降低了肌肉萎缩的关键因子 atrogin-1 和 MuRF-1 蛋白水平。此外，我们研究了鸡蛋成分，如亲和素、卵磷脂、生物素、3-sn-磷脂酰胆碱和 L-α-磷脂酰胆碱对地塞米松（DEX）处理的 C2C12 肌管的影响。蛋黄中的卵磷脂、生物素、3-sn-磷脂酰胆碱和 L-α-磷脂酰胆碱显著恢复了 DEX 应用后 C2C12 肌管直径的降低。亲和素没有显示出这种逆转。生物素、3-sn-磷脂酰胆碱和 L-α-磷脂酰胆碱也减弱了 DEX 增强的 atrogin-1 蛋白表达。我们的研究结果表明，蛋黄成分可能有助于逆转由肌肉损伤引起的骨骼肌萎缩。
