Shenzhen Medical Ultrasound Engineering Center, Department of Ultrasonography, Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen 518020, Guangdong, China.
Shenzhen Medical Ultrasound Engineering Center, Department of Ultrasonography, Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen 518020, Guangdong, China.
Int J Pharm. 2022 Mar 25;616:121299. doi: 10.1016/j.ijpharm.2021.121299. Epub 2021 Dec 17.
Traditional encapsulated microbubbles are recently used as delivery carriers for drugs and genes, but they have low efficiency. If the local microbubble concentration could be increased, this might be able to improve the therapeutic efficacy of diseases. In this study, we developed novel cationic magnetic microbubbles (MB), which could simultaneously realize targeted aggregation under a magnetic field as well as ultrasonographic real-time visualization. Their physicochemical properties, biocompatibility, ultrasonography, magnetic response characteristics, and biodistribution were systematically evaluated. Here, the MB were 2.55 ± 0.14 µm in size with a positive zeta potential, and had a good biocompatibility. They were able to enhance ultrasonographic contrast both in vitro and in vivo. MB could be attracted by an external magnet for directional movement and aggregation in vitro. We confirmed that MB also had a great magnetic response in vivo, by means of fluorescence imaging and contrast-enhanced ultrasound imaging. Following intravenous injection into tumor-bearing mice, MB showed excellent stability in the internal circulation, and could accumulate in the tumor vasculature through magnetic targeting. With the excellent combination of magnetic response and acoustic properties, cationic magnetic microbubbles (MB) have promising potential for use as a new kind of drug/gene carrier for theranostics in the future.
传统的包裹微泡最近被用作药物和基因的递送载体，但它们的效率较低。如果能增加局部微泡浓度，就有可能提高疾病的治疗效果。在这项研究中，我们开发了新型阳离子磁性微泡（MB），它可以在外磁场下同时实现靶向聚集和超声实时可视化。系统评估了它们的理化性质、生物相容性、超声、磁响应特性和体内分布。MB 的大小为 2.55±0.14μm，具有正的 Zeta 电位，具有良好的生物相容性。它们能够增强体外和体内的超声对比。MB 可以在外磁场的吸引下进行定向运动和体外聚集。我们通过荧光成像和对比增强超声成像证实，MB 在体内也具有很好的磁响应。静脉注射到荷瘤小鼠后，MB 在内部循环中表现出优异的稳定性，并可以通过磁靶向聚集在肿瘤血管中。阳离子磁性微泡（MB）具有优异的磁响应和声学特性相结合，有望成为治疗诊断学中一种新型药物/基因载体。
