Department of Botany, Panjab University, Chandigarh 160014, India.
Department of Environment Studies, Panjab University, Chandigarh 160014, India.
Ecotoxicol Environ Saf. 2022 Jan 1;229:113080. doi: 10.1016/j.ecoenv.2021.113080. Epub 2021 Dec 17.
Rice (Oryza sativa L.) is a highly consumed staple crop worldwide, but abiotic/heavy metal stresses acting on the plant cause reduction in yield and quality, thereby impacting global food security. In the present study, we examined the effect of β-pinene against Arsenic (As)-induced oxidative damage vis-à-vis regulation of activities of enzymatic antioxidants in roots of O. sativa. Effect of As (50 μM), β-pinene (10 μM; β-10) and As + β-10 treatments on root length, shoot length, As accumulation, lipid peroxidation (as malondialdehyde [MDA] content), hydrogen peroxide (HO) accumulation, and activities of lipoxygenase (LOX) and enzymatic antioxidants such as ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) was determined. Exposure of As caused a decline in root and shoot length, and enhancement in As accumulation, lipid peroxidation, and activities of enzymatic antioxidants. However, supplementation of β-10 (i.e., As + β-10 treatments) led to an increase in root and shoot length. Treatment with As + β-10 resulted in a decline in As accumulation, HO content, and MDA content; however, the effect on LOX activity was non-significant, as compared to control. Similarly, with As + β-10 treatment a reduction in the activities of APX, GPX, GR, SOD, and CAT was observed as compared with As-alone treatment. Pearson's correlation matrix exhibited strong negative correlation between reactive oxygen species (ROS) and root/shoot length, whereas a strong positive correlation was observed between antioxidant enzymes and ROS. The present study demonstrated that β-pinene significantly ameliorates As-induced oxidative stress and provides tolerance to O. sativa against As-induced toxicity, and thus offer an option of As-mitigation using environment friendly natural plant products. However, to gain insights into the function of β-pinene in modulating As-induced oxidative damage in plants, further field investigations and exploration of its mechanism of action are needed.
水稻（Oryza sativa L.）是全球广泛消费的主食作物，但植物受到的非生物/重金属胁迫会导致产量和质量下降，从而影响全球粮食安全。在本研究中，我们研究了β-蒎烯对砷（As）诱导的氧化损伤的影响，以及它对水稻根中酶抗氧化剂活性的调节作用。研究了 As（50 μM）、β-蒎烯（10 μM；β-10）和 As+β-10 处理对根长、茎长、As 积累、脂质过氧化（以丙二醛[MDA]含量表示）、过氧化氢（HO）积累、脂氧合酶（LOX）活性以及抗氧化酶如抗坏血酸过氧化物酶（APX）、愈创木酚过氧化物酶（GPX）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的影响。暴露于 As 会导致根长和茎长下降，As 积累、脂质过氧化和酶抗氧化剂活性增强。然而，β-10 的补充（即 As+β-10 处理）导致根长和茎长增加。As+β-10 处理导致 As 积累、HO 含量和 MDA 含量下降，而与对照相比，LOX 活性无显著变化。同样，与单独 As 处理相比，As+β-10 处理导致 APX、GPX、GR、SOD 和 CAT 活性降低。Pearson 相关矩阵显示，活性氧（ROS）与根/茎长之间呈强烈负相关，而抗氧化酶与 ROS 之间呈强烈正相关。本研究表明，β-蒎烯可显著减轻 As 诱导的氧化应激，提高水稻对 As 毒性的耐受性，从而为利用环保型天然植物产品减轻 As 污染提供了一种选择。然而，要深入了解β-蒎烯在调节植物中 As 诱导的氧化损伤方面的功能，还需要进一步的田间调查和对其作用机制的探索。
