Wang Ye, Wang Junfu, Wang Congjun, Chen Yeyang, Chen Junqiang
The First Affiliated Hospital of Guangxi Medical University, Department of Gastrointestinal Gland Surgery, Nanning, 530021, Guangxi, China; Guangxi Key Laboratory of Enhanced Recovery after Surgery for Gastrointestinal Cancer, Nanning, 530021, Guangxi, China.
The First Affiliated Hospital of Nanchang University, Department of General Surgery, Nanchang 330031, China.
Pathol Res Pract. 2022 Jan;229:153695. doi: 10.1016/j.prp.2021.153695. Epub 2021 Nov 20.
Papillary thyroid carcinoma (PTC) is one of the common clinical tumors, where LncRNA plays an important role in tumorigenesis and its development. The purpose of this study was to explore the role of DIO3OS in PTC.
Firstly, this study verified the expression of DIO3OS in PTC through the public database. Then, the differences in DIO3OS expression between the PTC group and paracancerous tissues were verified using the qRT-PCR. A series of in vitro experiments were conducted to verify the function of DIO3OS in PTC, while its involvement in possible pathways was analyzed by the GSEA. The ssGSEA algorithm estimated the immune status using the queue transcriptome graph derived from the TCGA database. Further, the correlation analysis was used to confirm the relationship between DIO3OS and the immune genes.
The results showed that the expression of DIO3OS was low in PTC. The same results were also confirmed by qRT-PCR analysis (P= 0.0077). In vitro, DIO3OS was localized within the cytoplasm and exosomes. Overexpression of DIO3OS hindered the proliferation, invasion, and migration of PTC cells. According to the degree of immune cell infiltration, the tumor group was divided into high immune cell infiltration group, medium immune cell infiltration group, and low immune cell infiltration group. The results showed that the DIO3OS was highly expressed in the high immune cell infiltration group (P < 0.001), which was positively correlated with the immune cell infiltration and also correlated with multiple immune genes.
In summary, this study illustrated the expression pattern of DIO3OS in PTC, which may be involved in the immune-inflammatory pathway. Hence, our results may provide new diagnostic biomarkers and therapeutic targets for PTC.
甲状腺乳头状癌（PTC）是常见的临床肿瘤之一，长链非编码RNA（LncRNA）在其肿瘤发生和发展中起重要作用。本研究旨在探讨DIO3OS在PTC中的作用。
首先，本研究通过公共数据库验证DIO3OS在PTC中的表达。然后，采用qRT-PCR验证PTC组与癌旁组织中DIO3OS表达的差异。进行了一系列体外实验以验证DIO3OS在PTC中的功能，同时通过基因集富集分析（GSEA）分析其可能涉及的途径。单样本基因集富集分析（ssGSEA）算法使用源自TCGA数据库的队列转录组图估计免疫状态。此外，采用相关性分析来确认DIO3OS与免疫基因之间的关系。
结果显示，PTC中DIO3OS的表达较低。qRT-PCR分析也证实了相同的结果（P = 0.0077）。在体外，DIO3OS定位于细胞质和外泌体中。DIO3OS的过表达阻碍了PTC细胞的增殖、侵袭和迁移。根据免疫细胞浸润程度，将肿瘤组分为高免疫细胞浸润组、中免疫细胞浸润组和低免疫细胞浸润组。结果显示，DIO3OS在高免疫细胞浸润组中高表达（P < 0.001），与免疫细胞浸润呈正相关，并且还与多个免疫基因相关。
总之，本研究阐明了DIO3OS在PTC中的表达模式，其可能参与免疫炎症途径。因此，我们的结果可能为PTC提供新的诊断生物标志物和治疗靶点。
