Department of Neurology and Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China.
Department of Neurology, First Affiliated Hospital of Hainan Medical University, Haikou, China.
J Clin Neurosci. 2022 Jan;95:75-80. doi: 10.1016/j.jocn.2021.11.020. Epub 2021 Dec 9.
Interleukin 35 (IL-35) plays an anti-inflammatory in numerous autoimmune diseases. However, the potential roles of IL-35-producing T and B cells and serum IL-35 levels in the pathogenesis of myasthenia gravis (MG) and its association with disease activity in patients with MG remain unclear.
The percentages of IL-35-producing CD4 + CD25 + T cells and CD19 + B cells among peripheral blood mononuclear cells were determined in 37 patients with anti-acetylcholine receptor (AChR) antibody-positive MG and 35 healthy controls (HCs) by performing a flow cytometry analysis. Serum IL-35 levels in participants were determined using an enzyme-linked immunosorbent assay. Further, the correlations between IL35 levels and disease activity were analysed.
The percentages of IL-35-producing CD4 + CD25 + T cells and CD19 + B cells were significantly lower in patients with anti-AChR antibody-positive MG than in HCs (p = 0.001 and p = 0.002, respectively). Furthermore, patients with thymoma and patients with generalized MG had lower percentages of IL-35-producing CD4 + CD25 + T cells and CD19 + B cells than those without thymoma and those with ocular MG (p = 0.001 and p = 0.003; p = 0.008 and p = 0.001, respectively). Interestingly, the suppression of IL-35 secretion correlated negatively with the activities of daily living scores of patients with MG (r = -0.4774, p = 0.0028) and the quantitative MG scores (r = -0.4656, p = 0.0037). The proportions of IL-35-producing T cells and B cells and serum levels of IL-35 increased after treatment.
IL-35 may represent a potential biomarker for the clinical evaluation of MG.
白细胞介素 35 (IL-35) 在许多自身免疫性疾病中发挥抗炎作用。然而，IL-35 产生的 T 和 B 细胞以及血清 IL-35 水平在重症肌无力 (MG) 发病机制中的潜在作用及其与 MG 患者疾病活动的关系尚不清楚。
通过流式细胞术分析，测定 37 例抗乙酰胆碱受体 (AChR) 抗体阳性 MG 患者和 35 例健康对照者 (HCs) 外周血单个核细胞中 IL-35 产生的 CD4+CD25+T 细胞和 CD19+B 细胞的百分比。采用酶联免疫吸附试验测定参与者的血清 IL-35 水平。进一步分析了 IL35 水平与疾病活动的相关性。
抗 AChR 抗体阳性 MG 患者 IL-35 产生的 CD4+CD25+T 细胞和 CD19+B 细胞的百分比明显低于 HCs（p=0.001 和 p=0.002）。此外，胸腺瘤患者和全身性 MG 患者的 IL-35 产生的 CD4+CD25+T 细胞和 CD19+B 细胞百分比低于无胸腺瘤和眼肌型 MG 患者（p=0.001 和 p=0.003；p=0.008 和 p=0.001）。有趣的是，IL-35 分泌的抑制与 MG 患者的日常生活活动评分（r=-0.4774，p=0.0028）和定量 MG 评分（r=-0.4656，p=0.0037）呈负相关。治疗后，IL-35 产生的 T 细胞和 B 细胞的比例以及血清 IL-35 水平增加。
IL-35 可能是 MG 临床评估的潜在生物标志物。
