Chiu K M, Knospe W H
Exp Hematol. 1987 Mar;15(3):269-75.
Experimental aplastic anemia (EAA) can be induced in CBA/J mice when they are sublethally irradiated and injected with lymph node cells (LNC) from C3H/He mice. Mice injected with LNC die of severe pancytopenia and marrow aplasia. In the present study, cells from other anatomical locations and subsets of LNC were examined for their ability to induce and to modulate EAA. Of peritoneal, splenic, and thymic cells, only cells from the thymus had EAA activity. C3H/He bone marrow cells did not induce any adverse effects in sublethally or lethally irradiated CBA/J mice. LNC, when depleted of B or phagocytic cells, retained EAA activity. In contrast, LNC depleted of T cells had significantly less EAA activity. Furthermore, when T cells of LNC were separated into peanut agglutinin (PNA)-receptor-positive and negative fractions, only the PNA- cells were able to induce EAA. EAA activity was lost when LNC were irradiated (1000 rad). The inability of splenic or bone marrow cells to induce EAA could have been due to the presence of cells that suppressed EAA activity. When splenic or bone marrow cells were coinjected with LNC, EAA was not induced. Coinjected irradiated splenic cells, but not bone marrow cells, were still able to inhibit EAA activity. Bone marrow cells seemed to inhibit EAA by replacing stem cells that were lost during the EAA process. On the other hand, splenic cells appeared to suppress EAA activity of LNC. Thus, radiosensitive PNA- T cells of lymph nodes or thymus were capable of inducing EAA, and their activity could be modulated by radioresistant splenic cells.
当CBA/J小鼠接受亚致死剂量照射并注射来自C3H/He小鼠的淋巴结细胞（LNC）时，可诱导出实验性再生障碍性贫血（EAA）。注射LNC的小鼠死于严重的全血细胞减少和骨髓再生障碍。在本研究中，检测了来自其他解剖部位的细胞和LNC亚群诱导和调节EAA的能力。在腹膜、脾脏和胸腺细胞中，只有来自胸腺的细胞具有EAA活性。C3H/He骨髓细胞在亚致死或致死剂量照射的CBA/J小鼠中未引起任何不良反应。LNC去除B细胞或吞噬细胞后仍保留EAA活性。相比之下，去除T细胞的LNC的EAA活性显著降低。此外，当LNC的T细胞被分离为花生凝集素（PNA）受体阳性和阴性部分时，只有PNA阴性细胞能够诱导EAA。LNC经1000拉德照射后EAA活性丧失。脾脏或骨髓细胞不能诱导EAA可能是由于存在抑制EAA活性的细胞。当脾脏或骨髓细胞与LNC共同注射时，未诱导出EAA。共同注射的经照射的脾脏细胞而非骨髓细胞仍能抑制EAA活性。骨髓细胞似乎通过替代EAA过程中丢失的干细胞来抑制EAA。另一方面，脾脏细胞似乎抑制LNC的EAA活性。因此，淋巴结或胸腺的放射敏感性PNA阴性T细胞能够诱导EAA，其活性可被放射抗性的脾脏细胞调节。
