Corps A N, Blakeley D M, Carr J, Rees L H, Brown K D
J Endocrinol. 1987 Jan;112(1):151-9. doi: 10.1677/joe.0.1120151.
The concentration of epidermal growth factor (EGF) in human mammary secretions was about 50 nmol/l for several weeks prepartum. It then fell to about 13 nmol/l within 4 days after parturition, in parallel with the decrease in protein concentration which is associated with the onset of lactation. In contrast, the concentration of EGF in urine samples from the same donors remained constant throughout this period. All the immunoreactive EGF in mammary secretions competed at the EGF receptors on Swiss mouse 3T3 fibroblasts. The stimulation of these cells by samples of mammary secretions was, however, much greater than that induced by EGF alone, indicating the presence of other factors which synergize with EGF. Gel filtration of mammary secretions revealed two major peaks of mitogenic activity, corresponding to EGF and a factor of higher molecular weight. The latter could synergize with added EGF, insulin or bombesin, and thus falls into a different functional class from any of these factors.
在产前数周，人乳分泌物中表皮生长因子（EGF）的浓度约为50 nmol/L。产后4天内，其浓度降至约13 nmol/L，与此同时，与泌乳开始相关的蛋白质浓度也降低。相比之下，同一供体的尿液样本中EGF的浓度在此期间保持恒定。乳分泌物中的所有免疫反应性EGF都能与瑞士小鼠3T3成纤维细胞上的EGF受体竞争。然而，乳分泌物样本对这些细胞的刺激作用远大于单独的EGF所诱导的作用，这表明存在与EGF协同作用的其他因子。对乳分泌物进行凝胶过滤显示出两个主要的促有丝分裂活性峰，分别对应于EGF和一个分子量更高的因子。后者可与添加的EGF、胰岛素或蛙皮素协同作用，因此属于与这些因子不同的功能类别。
