Schnyder J, Baggiolini M, Schreier M H
Agents Actions. 1986 Dec;19(5-6):277-9. doi: 10.1007/BF01971228.
Stimulation of macrophages by zymosan phagocytosis triggers the respiratory burst and induces an early release of lysosomal hydrolases and E-type prostaglandins (PGE). We have studied whether antigen presentation by macrophages to helper T-cells elicits a comparable sequence of events. Cloned T-helper cells specific for hen egg albumin (EA) were added to histocompatible or histoincompatible resident mouse peritoneal macrophages in the presence of EA or an unrelated antigen, and the changes in biochemical parameters were monitored. The interaction between macrophages. T-helper cells and EA induced the production of PGE, but no release of lysosomal hydrolases or activation of the respiratory burst. In addition T-cell proliferation was observed. By contrast, no proliferation and no biochemical changes were observed when histoincompatible macrophages or unrelated antigen were used. When the experiments were done in the presence of indomethacin to inhibit PGE release, T-cell proliferation was enhanced. These results suggest that the PGE released may exert a feed-back control of the T-cell response.
酵母聚糖吞噬作用刺激巨噬细胞会引发呼吸爆发，并诱导溶酶体水解酶和E型前列腺素（PGE）的早期释放。我们研究了巨噬细胞向辅助性T细胞呈递抗原是否会引发类似的一系列事件。将对鸡卵清蛋白（EA）特异的克隆辅助性T细胞，在EA或无关抗原存在的情况下，加入到组织相容性或组织不相容的驻留小鼠腹腔巨噬细胞中，并监测生化参数的变化。巨噬细胞、辅助性T细胞和EA之间的相互作用诱导了PGE的产生，但没有溶酶体水解酶的释放或呼吸爆发的激活。此外，还观察到了T细胞增殖。相比之下，当使用组织不相容的巨噬细胞或无关抗原时，未观察到增殖和生化变化。当在吲哚美辛存在下进行实验以抑制PGE释放时，T细胞增殖增强。这些结果表明，释放的PGE可能对T细胞反应发挥反馈控制作用。
