van Loveren H R, Haas H L, Greene R W, Siegfried J
Appl Neurophysiol. 1986;49(3):147-54. doi: 10.1159/000100139.
This report describes a simple in vitro method to harvest and study the electrophysiological properties of the trigeminal ganglion of the rat. In suction electrode recordings from the proximal nerve end, two distinct peaks are identified in the compound action potential evoked by electrical stimulation of the distal nerve. gamma-Aminobutyric acid (GABA), added to the perfusion fluid, caused a positive shift in the DC potential (depolarization) and a partially selective decrease in the amplitude of the slow-conduction peak. Reversible antagonism of the GABA effect by picrotoxin and bicuculline suggests that both responses are receptor mediated. There was no response to carbamazepine or L-baclofen, suggesting that ganglionic polarization does not play a major role in the action of these drugs in trigeminal neuralgia.
本报告描述了一种简单的体外方法，用于获取和研究大鼠三叉神经节的电生理特性。在从近端神经末梢进行的吸力电极记录中，电刺激远端神经诱发的复合动作电位中有两个不同的峰值。添加到灌流液中的γ-氨基丁酸（GABA）导致直流电位正向偏移（去极化），并使慢传导峰值的幅度部分选择性降低。印防己毒素和荷包牡丹碱对GABA效应的可逆拮抗作用表明，这两种反应均由受体介导。卡马西平和L-巴氯芬无反应，表明神经节极化在这些药物治疗三叉神经痛的作用中不起主要作用。
