Kimoto Y, Tanji Y, Tanaka T, Taguchi T
Gan To Kagaku Ryoho. 1987 Mar;14(3 Pt 1):687-92.
Peripheral blood lymphocytes obtained from patients by leukapheresis were cultured in RPMI 1640 containing human plasma and interleukin 2. The morphology, phenotypes and cytotoxicity of induced LAK cells were studied. Lymphoblastoid cells mainly proliferated were OKIa1+ cells and were thought to be LAK cells. Maximal cytotoxicity was obtained after two weeks of incubation. IL-2 enhanced the cytotoxicity of LAK cells. Autologous LAK cells induced by two weeks of incubation were injected into patients. One case of pulmonary metastases of breast cancer showed reduction and two lesions showed partial regression. Also, no new lesions appeared in the lungs of a patient with alveolar soft-part sarcoma.
通过白细胞分离术从患者获取的外周血淋巴细胞，在含有人类血浆和白细胞介素2的RPMI 1640培养基中培养。对诱导的LAK细胞的形态、表型和细胞毒性进行了研究。主要增殖的淋巴母细胞样细胞为OKIa1 +细胞，被认为是LAK细胞。孵育两周后获得最大细胞毒性。白细胞介素2增强了LAK细胞的细胞毒性。将孵育两周诱导的自体LAK细胞注入患者体内。1例乳腺癌肺转移患者的转移灶缩小，2个病灶部分消退。此外，1例肺泡软组织肉瘤患者的肺部未出现新病灶。
