Seo Young-Jun, Lim Byeonghwi, Kim Do-Young, Lim Kyu-Sang, Kim Jun-Mo
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Gyeonggi-do, Korea.
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Animals (Basel). 2021 Dec 9;11(12):3511. doi: 10.3390/ani11123511.
Recently, interest in the function of pig backfat (BF) has increased in the field of livestock animals, and many transcriptome-based studies using commercial pig breeds have been conducted. However, there is a lack of comprehensive studies regarding the biological mechanisms of Korean native pigs (KNPs) and Yorkshire pig crossbreeds. In this study, therefore, BF samples of F1 crossbreeds of KNPs and Yorkshire pigs were investigated to identify differentially expressed genes (DEGs) and their related terms using RNA-sequencing analysis. DEG analysis identified 611 DEGs, of which 182 were up-regulated and 429 were down-regulated. Lipid metabolism was identified in the up-regulated genes, whereas growth and maturation-related terminologies were identified in the down-regulated genes. and were identified as highly connected core genes during functional gene network analysis. Fat tissue was observed to affect lipid metabolism and organ development due to hormonal changes driven by transcriptional alteration. This study provides a comprehensive understanding of BF contribution to crossbreeds of KNPs and Yorkshire pigs during growth periods.
近年来，家畜领域对猪背膘（BF）功能的关注度不断提高，并且已经开展了许多基于转录组的商业猪品种研究。然而，关于韩国本土猪（KNP）与约克夏猪杂交品种的生物学机制，目前仍缺乏全面的研究。因此，在本研究中，通过RNA测序分析，对KNP与约克夏猪的F1杂交品种的BF样本进行了研究，以鉴定差异表达基因（DEG）及其相关术语。差异表达基因分析共鉴定出611个DEG，其中182个上调，429个下调。上调基因中鉴定出脂质代谢相关基因，而下调基因中鉴定出与生长和成熟相关的术语。在功能基因网络分析中，[具体基因名称1]和[具体基因名称2]被确定为高度连接的核心基因。研究发现，转录改变驱动的激素变化会使脂肪组织影响脂质代谢和器官发育。本研究全面揭示了BF在KNP与约克夏猪杂交品种生长阶段所起的作用。
