Bakaletz L O, DeMaria T F, Lim D J
Arch Otolaryngol Head Neck Surg. 1987 May;113(5):526-9. doi: 10.1001/archotol.1987.01860050072018.
Because bacteria are known to persist in middle ear effusions for extended periods of time despite the presence of increasing titers of specific antibody in both sera and middle ear fluids in humans, we examined the ability of postinfection serum to opsonize Haemophilus influenzae (nontypable and type b) for phagocytosis by middle ear macrophages in chinchillas. Our data indicated that preopsonization of both strains of H influenzae enhanced phagocytosis by macrophages recruited to the middle ear following intrabullar instillation of keyhole limpet hemocyanin. Both the total number of bacteria ingested and the percentage of macrophages phagocytizing were significantly greater in the presence of postinfection serum than preinfection serum for all animals tested.
尽管人类血清和中耳液中特异性抗体滴度不断升高，但已知细菌仍会在中耳积液中长时间持续存在。因此，我们检测了感染后血清调理流感嗜血杆菌（非b型和b型）以便让豚鼠中耳巨噬细胞进行吞噬的能力。我们的数据表明，两种流感嗜血杆菌菌株的预调理增强了在鼓室内注入钥孔戚血蓝蛋白后募集到中耳的巨噬细胞的吞噬作用。对于所有受试动物，在存在感染后血清的情况下，摄入的细菌总数和吞噬细菌的巨噬细胞百分比均显著高于感染前血清。
