Shanahan W R, Padula S J, Clark R B, Korn J H
Clin Immunol Immunopathol. 1987 May;43(2):204-10. doi: 10.1016/0090-1229(87)90128-0.
Recent studies suggest a role for endothelial cells (EC) and fibroblasts (FB) in performing certain accessory functions of monocytes in immune responses. We examined the ability of allogeneic adherent cells (AC), umbilical vein EC, and foreskin FB to support antigen and mitogen responses of tetanus toxoid-responsive human T-cell lines (TCTet). Syngeneic AC supported antigen and mitogen (phytohemagglutinin and pokeweed mitogen, PHA and PWM) responses of TCTet. Allogeneic AC, EC, and FB supported mitogen but not antigen responses of TCTet, in a dose-dependent manner. PHA-activated mononuclear cell supernates or EC or FB supernates could not replace accessory cells in mitogen responses. We provide further evidence that EC and FB can function as fully competent accessory cells, a function that may be of significance in in vivo initiation of immune responses.
近期研究表明，内皮细胞（EC）和成纤维细胞（FB）在免疫反应中执行单核细胞的某些辅助功能方面发挥作用。我们检测了同种异体贴壁细胞（AC）、脐静脉EC和包皮FB支持破伤风类毒素反应性人T细胞系（TCTet）的抗原和丝裂原反应的能力。同基因AC支持TCTet的抗原和丝裂原（植物血凝素和商陆丝裂原，PHA和PWM）反应。同种异体AC、EC和FB以剂量依赖方式支持TCTet的丝裂原反应，但不支持抗原反应。PHA激活的单核细胞上清液或EC或FB上清液在丝裂原反应中不能替代辅助细胞。我们提供了进一步的证据表明，EC和FB可作为完全有功能的辅助细胞发挥作用，这一功能在体内免疫反应启动中可能具有重要意义。
