Fukushi M, Arai O, Mizushima Y, Takasugi N, Fujieda K, Matsuura N
Nihon Naibunpi Gakkai Zasshi. 1987 Feb 20;63(2):113-22.
An enzyme-linked immunosorbent assay (ELISA) for 17 alpha-hydroxyprogesterone (17-OHP) in dried blood collected on filter paper has been developed. The ELISA method is very easy and rapid and has sensitivity, accuracy and precision. The detection limit of the ELISA method is 0.5 ng/ml blood. Intra- and interassay coefficients of variation are 7.0 approximately 8.3% and 6.7 approximately 10.5%, respectively. Correlation between the ELISA method and previously reported enzyme immunoassay is good (r = 0.961). 17-OHP values of neonates with congenital adrenal hyperplasia (CAH) are extremely high compared with normal neonates (11.0 +/- 5.9 ng/ml blood). The present study demonstrates that the ELISA method for 17-OHP is applicable to neonatal screening for CAH.
已开发出一种用于检测滤纸上采集的干血中17α-羟孕酮（17-OHP）的酶联免疫吸附测定（ELISA）法。ELISA方法非常简便、快速，且具有灵敏度、准确性和精密度。ELISA法的检测限为0.5 ng/ml血液。批内和批间变异系数分别约为7.0%至8.3%和6.7%至10.5%。ELISA法与先前报道的酶免疫测定法之间的相关性良好（r = 0.961）。与正常新生儿（11.0±5.9 ng/ml血液）相比，先天性肾上腺增生（CAH）新生儿的17-OHP值极高。本研究表明，用于17-OHP的ELISA法适用于CAH的新生儿筛查。
