Biospectrum Life Science Institute, A-1805, U-TOWER, Yongin-si 16827, Korea.
Basic Research and Innovation Division, Amorepacific Corporation R&D Center, Youngin-si 17074, Korea.
Int J Mol Sci. 2021 Dec 20;22(24):13673. doi: 10.3390/ijms222413673.
Functional studies of organisms and human models have revealed that epigenetic changes can significantly impact the process of aging. Non-coding RNA (ncRNA), one of epigenetic regulators, plays an important role in modifying the expression of mRNAs and their proteins. It can mediate the phenotype of cells. It has been reported that nc886 (=vtRNA2-1 or pre-miR-886), a long ncRNA, can suppress tumor formation and photo-damages of keratinocytes caused by UVB. The aim of this study was to determine the role of nc886 in replicative senescence of fibroblasts and determine whether substances capable of controlling nc886 expression could regulate cellular senescence. In replicative senescence fibroblasts, nc886 expression was decreased while methylated nc886 was increased. There were changes of senescence biomarkers including SA-β-gal activity and expression of p16INK4A and p21Waf1/Cip1 in senescent cells. These findings indicate that the decrease of nc886 associated with aging is related to cellular senescence of fibroblasts and that increasing nc886 expression has potential to suppress cellular senescence. AbsoluTea Concentrate 2.0 (ATC) increased nc886 expression and ameliorated cellular senescence of fibroblasts by inhibiting age-related biomarkers. These results indicate that nc886 has potential as a new target for anti-aging and that ATC can be a potent epigenetic anti-aging ingredient.
功能研究生物体和人类模型表明，表观遗传变化可显著影响衰老过程。非编码 RNA（ncRNA）作为表观遗传调控因子之一，在修饰 mRNA 及其蛋白的表达方面发挥重要作用。它可以调节细胞的表型。据报道，nc886（=vtRNA2-1 或 pre-miR-886），一种长 ncRNA，可以抑制肿瘤的形成和紫外线 B（UVB）引起的角质形成细胞的光损伤。本研究旨在确定 nc886 在成纤维细胞复制性衰老中的作用，并确定是否能够控制 nc886 表达的物质可以调节细胞衰老。在复制性衰老的成纤维细胞中，nc886 的表达减少，而甲基化 nc886 增加。衰老标志物如 SA-β-半乳糖苷酶活性以及 p16INK4A 和 p21Waf1/Cip1 的表达都发生了变化。这些发现表明，与衰老相关的 nc886 的减少与成纤维细胞的细胞衰老有关，并且增加 nc886 的表达有可能抑制细胞衰老。AbsoluTea Concentrate 2.0（ATC）通过抑制与年龄相关的生物标志物，增加 nc886 的表达并改善成纤维细胞的衰老。这些结果表明 nc886 具有作为抗衰老新靶标的潜力，并且 ATC 可以作为一种有效的表观遗传抗衰老成分。
