miRNA-490-3p 通过靶向 AURKA 抑制胃癌细胞生长。

Targeting AURKA by microRNA-490-3p suppresses gastric cancer cell growth.

机构信息

Department of Gastroenterology, Taizhou Hospital of Zhejiang Province, Taizhou, Zhejiang, PR China.

Department of Emergency, Taizhou Hospital of Zhejiang Province, Taizhou, Zhejiang, PR China.

出版信息

Histol Histopathol. 2022 Apr;37(4):397-404. doi: 10.14670/HH-18-415. Epub 2021 Dec 24.

DOI:10.14670/HH-18-415

PMID:34951005

Abstract

OBJECTIVE

To illustrate the molecular mechanism of microRNA-490-3p regulating gastric cancer (GC) cells by targeting AURKA.

METHODS

Genes with significantly different expression in GC and normal tissue in TCGA-STAD dataset were analyzed by bioinformatics. Expression levels of genes and proteins in GC cells were measured by qRT-PCR and western blot. The interaction between microRNA-490-3p and AURKA was verified by dual luciferase assay. Proliferation, migration, invasion and apoptosis of GC cells were evaluated through a set of cell function assays.

RESULTS

MicroRNA-490-3p was significantly less expressed in GC, while AURKA was significantly highly expressed. Dual luciferase reporter gene assay proved that microRNA-490-3p targeted AURKA. Up-regulation of microRNA-490-3p restrained proliferation, migration, invasion and stimulated apoptosis of GC cells, which was attenuated by overexpression of AURKA.

CONCLUSIONS

MicroRNA-490-3p was likely to restrain the development of GC cells by inhibiting AURKA, and it may be an underlying target for GC treatment.

摘要

目的

通过靶向 AURKA 来阐明 microRNA-490-3p 调控胃癌（GC）细胞的分子机制。

方法

通过生物信息学分析 TCGA-STAD 数据集，分析 GC 和正常组织中差异显著表达的基因。采用 qRT-PCR 和 Western blot 检测 GC 细胞中基因和蛋白的表达水平。通过双荧光素酶报告基因实验验证 microRNA-490-3p 与 AURKA 之间的相互作用。通过一系列细胞功能实验评估 GC 细胞的增殖、迁移、侵袭和凋亡。