Department of General Surgery, Tonglu First People's Hospital, No.338 Xuesheng Road, Hangzhou, Zhejiang, 311500, People's Republic of China.
Appl Biochem Biotechnol. 2022 Sep;194(9):3833-3842. doi: 10.1007/s12010-022-03944-2. Epub 2022 May 11.
MicroRNAs are reported to be involved in tumor development. This study aims to investigate the biological functions and molecular mechanisms of microRNA-19a-3p in gastric cancer cells. TCGA-based expression analysis and qRT-PCR assay illustrated that microRNA-19a-3p was overexpressed in gastric cancer. MTT and Transwell assays indicated that microRNA-19a-3p could strengthen the proliferation, migration, and invasion of gastric cancer cells. SMOC2 was bioinformatically predicted as the target of microRNA-19a-3p, followed by identified using a dual-luciferase assay. The effects of microRNA-19a-3p/SMOC2 regulatory axis on gastric cancer cells were examined by MTT and Transwell assays as well. Concludingly, this study demonstrated that microRNA-19a-3p could promote the aggressive cell phenotypes of gastric cancer cells by targeting SMOC2.
微 RNA 被报道参与肿瘤的发生发展。本研究旨在探讨微 RNA-19a-3p 在胃癌细胞中的生物学功能和分子机制。基于 TCGA 的表达分析和 qRT-PCR 实验表明，微 RNA-19a-3p 在胃癌中呈过表达。MTT 和 Transwell 实验表明，微 RNA-19a-3p 可以增强胃癌细胞的增殖、迁移和侵袭能力。SMOC2 被生物信息学预测为 microRNA-19a-3p 的靶基因，随后通过双荧光素酶报告基因实验进行验证。通过 MTT 和 Transwell 实验进一步研究了 microRNA-19a-3p/SMOC2 调控轴对胃癌细胞的影响。综上所述，本研究表明，microRNA-19a-3p 可以通过靶向 SMOC2 促进胃癌细胞的侵袭表型。
