Knowles P P, Thorpe P E
Anal Biochem. 1987 Feb 1;160(2):440-3. doi: 10.1016/0003-2697(87)90073-x.
We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.
我们描述了一种通过在偶联到琼脂糖的汽巴蓝F3GA（蓝色琼脂糖）上进行亲和层析从免疫毒素中分离抗体的方法。抗体不与凝胶结合。免疫毒素通过其蓖麻毒素A链或相思子毒素A链部分结合，并且可以使用温和的洗脱条件以高产量和高纯度回收。该方法适用于免疫毒素的大规模纯化。
